Prevalence and Antibiotic Resistance Patterns of Salmonella Isolates from Lactating Cows andIn-contact Humans in Dairy Farms, Northwest Ethiopia -

D. Hailu, A. Gelaw, W. Molla, L. Garedew, L. Cole, Roger P. Johnson
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引用次数: 12

Abstract

Background: Salmonellosis is an infectious disease of humans and animals caused by species of Salmonella. Salmonella are widespread in the environment and commonly found in farm effluents and sewage. Epidemiological information on the prevalence of salmmonelosis is a prerequisite to develop appropriate control strategies. Objective: To assess the prevalence and antibiotic resistance patterns of Salmonella isolates. Methods: A cross-sectional study was conducted. A total of 152 cows and 14 humans were included in the study. Milk, fecal sample and human stool were collected. Samples were pre-enriched with buffered peptone water and transferred to selenite cysteine broth and rappaport-vassilidis broth. Isolation was made by plating on to xylose lysine deoxycholate agar and brilliant green agar. Biochemical, antibiotic susceptibility testing and serotyping were done. Data were entered and analyzed using SPSS version 20 software. Binary logistic regression was applied and p-values of less than 0.05 were considered statistically significant. Results: Of the total 21 Salmonella isolates identified, 19(90.5%) and 2(9.5%) isolates were from lactating cows and in-contact individuals, respectively. Salmonella bredeney was the most common (85.7%). Drinking water source had statistically significant (P=0.027) association. All isolates were resistant at least to one of the antibiotics tested with 100% to ampicillin. All isolates were sensitive to ciprofloxacin and amoxacilin/clavulanic acid. Conclusion: Large percentage of Salmonella isolates were resistant at least to one of the antibiotics tested. Salmonellosis treatment could be difficult in the future unless wise use of antibiotics is practiced.
埃塞俄比亚西北部奶牛场产乳牛和接触者分离的沙门氏菌流行率和抗生素耐药性模式
背景:沙门氏菌病是一种由沙门氏菌引起的人畜传染病。沙门氏菌在环境中广泛存在,通常存在于农场废水和污水中。关于沙门氏菌病流行的流行病学信息是制定适当控制战略的先决条件。目的:了解沙门氏菌的流行情况及耐药模式。方法:采用横断面研究。研究对象是152头牛和14人。采集牛奶、粪便和人粪便样本。样品用缓冲蛋白胨水预先富集,并转移到亚硒酸盐半胱氨酸肉汤和rappaport-vassilidis肉汤中。用木糖赖氨酸脱氧胆酸琼脂和亮绿琼脂进行分离。进行生化、药敏及血清分型检测。使用SPSS 20版软件进行数据录入和分析。采用二元逻辑回归,p值小于0.05认为有统计学意义。结果:共鉴定出21株沙门氏菌,其中19株(90.5%)来自乳牛,2株(9.5%)来自接触者。其中以布氏沙门氏菌最多见(85.7%)。饮用水源相关性有统计学意义(P=0.027)。所有分离株至少对一种抗生素耐药,对氨苄西林的耐药率为100%。所有分离株均对环丙沙星和阿莫西林/克拉维酸敏感。结论:大部分分离的沙门氏菌至少对一种抗生素耐药。除非明智地使用抗生素,否则沙门氏菌病的治疗在未来可能会很困难。
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