Effect of vestibular stimulation on cold water stress-induced neurological changes in Wistar rats

Rama Kranthi T, Archana R, Senthilkumar Sivanesan
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Abstract

The current study was undertaken to see the effects of cold-water stress on the brain and to evaluate the beneficial effect of vestibular stimulation on stress-induced brain changes. Healthy, male, Wistar rats, weighing 180 to 250 gm with 3-6 months of age, were used for the study. Stress was induced by making the animals swim in cold water maintained at 100C for 30 min a day, for 14 days. Following cold water swimming stress, bilateral hot water caloric vestibular stimulation was given to the animals using 410C temperature water for 15 days. Rats were sacrificed and histopathological brain changes were studied by Hematoxylin & Eosin staining. Serum corticosterone level has increased significantly after cold water swimming stress (p<0.01). Corticosterone was less in animals that received caloric vestibular stimulation in comparison with the animals which did not receive caloric vestibular stimulation (p<0.05). Coldwater swimming stress had induced focal neuronal atrophy, nuclear pyknosis with congested blood vessels and infiltration of mononuclear inflammatory cells in the hippocampus and hypothalamus. Stressed animals that received caloric vestibular stimulation recovered well and showed the cerebral cortex with the normal neuroglial arrangement. Hypothalamus showed normal morphology and the hippocampus showed a pyramidal layer with a normal thickness in comparison to the animals which did not receive caloric vestibular stimulation. We conclude Caloric vestibular stimulation was effective in reversing the cold-water stress-induced serum corticosterone and histopathological changes in the brain.
前庭刺激对冷水应激诱导Wistar大鼠神经变化的影响
目前的研究是为了观察冷水应激对大脑的影响,并评估前庭刺激对应激引起的大脑变化的有益影响。本研究选用健康雄性Wistar大鼠,体重180 ~ 250克,3 ~ 6月龄。在100℃的冷水中游泳,每天30分钟,连续14天。在冷水游泳应激后,采用410℃热水对动物进行双侧前庭热刺激,持续15天。处死大鼠,苏木精和伊红染色观察脑组织病理变化。冷水游泳应激后血清皮质酮水平显著升高(p<0.01)。与未接受前庭热刺激的动物相比,接受前庭热刺激的动物皮质酮含量较低(p<0.05)。冷水游泳应激引起局灶性神经元萎缩,核固缩伴血管充血,海马和下丘脑单核炎性细胞浸润。接受前庭热刺激的应激动物恢复良好,大脑皮层神经胶质排列正常。与未接受前庭热刺激的动物相比,下丘脑形态正常,海马呈锥体层,厚度正常。我们得出结论,热量前庭刺激在逆转冷水应激诱导的血清皮质酮和大脑组织病理学变化方面是有效的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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