Effect of Glytan on Liver Fibrosis in Portal Hypertensive Rats

Abstract

The aim of this research was to observe the effect of Glytan on rat liver fibrosis and to investigate the mechanism of portal hypertension (PHT). SD male rats with weight between 240 g and 260 g were randomly divided into a sham group, model group, propranolol group, and Glytan group, according to their weight. PHT and liver fibrosis were induced by common bile duct ligation. After 2 and 4 weeks, the hydroxyproline (Hyp) content of liver tissues was tested by spectrophotometer. The collagen formation was evaluated by Masson staining and ultrastructural changes were evaluated by transmission electron microscopy (TEM). The results showed that Glytan inhibited the Hyp production at 2 weeks and 4 weeks. Masson staining revealed the same results with Hyp. Glytan inhibited the pseudolobule formation. TEM showed swelling sinusoid endothelial cells (SEC), reduced fenestra between the SEC, widened Disse's space, and reduced hepatocyte microvilli in the model group rats. At 4 weeks, loss of the hepatocyte microvilli and the basement membrane were clearly seen. Glytan promoted degeneration of the matrix and restoration of the SEC at 2 weeks and 4 weeks. It was concluded that Glytan inhibited collagen formation and ameliorated sinusoidal capillarization. This is one of the mechanisms of Glytan used to decrease the portal venous pressure. The results provided precise and reliable proof to develop new treatments for PHT.