MSM Supplementation Is Associated with Reduced Inflammation and Improved Innate Immune Response following In Vitro LPS-Stimulation in Humans after a Bout of Downhill Running

IF 0.5 Q4 ORTHOPEDICS

MLTJ-Muscles Ligaments and Tendons Journal Pub Date : 2023-05-06 DOI:10.3390/muscles2020015

B. McFarlin, J. Vingren, D. Hill, Elizabeth A. Bridgeman

{"title":"MSM Supplementation Is Associated with Reduced Inflammation and Improved Innate Immune Response following In Vitro LPS-Stimulation in Humans after a Bout of Downhill Running","authors":"B. McFarlin, J. Vingren, D. Hill, Elizabeth A. Bridgeman","doi":"10.3390/muscles2020015","DOIUrl":null,"url":null,"abstract":"Exercise-induced muscle injury and the subsequent release of Damage-Associated Molecular Patterns (DAMP) result in soreness and inflammation. Dietary supplements may accelerate the rate of recovery by supporting resolution of inflammation. The purpose of this study was to determine if methylsulfonylmethane (MSM) supplementation (30 d prior to exercise and during recovery) altered mRNA expression in LPS-exposed blood leukocytes after a bout of downhill running. Exercise consisted of 60 min of downhill running (−15% grade). Blood (baseline, pre-exercise, 4, 24, 48, and 72 h post-exercise) was diluted (1:10) and combined with LPS (20 µg/mL) for 24 h. Total RNA was isolated from leukocytes and analyzed for 574 immune-associated mRNA (Nanostring nCounter; ROSALIND.BIO). Data were expressed as log2 fold change from baseline for each condition (MSM and placebo). Compared to placebo, MSM supplementation was associated with an improved inflammation response (15 mRNA) and viral immune response (2 mRNA). The largest number of changes were found at 4 and 24 h post-exercise. The key finding in the present study is that MSM supplementation can improve inflammation management and the innate immune response after exercise.","PeriodicalId":46318,"journal":{"name":"MLTJ-Muscles Ligaments and Tendons Journal","volume":"87 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2023-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"MLTJ-Muscles Ligaments and Tendons Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/muscles2020015","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ORTHOPEDICS","Score":null,"Total":0}

引用次数: 0

Abstract

Exercise-induced muscle injury and the subsequent release of Damage-Associated Molecular Patterns (DAMP) result in soreness and inflammation. Dietary supplements may accelerate the rate of recovery by supporting resolution of inflammation. The purpose of this study was to determine if methylsulfonylmethane (MSM) supplementation (30 d prior to exercise and during recovery) altered mRNA expression in LPS-exposed blood leukocytes after a bout of downhill running. Exercise consisted of 60 min of downhill running (−15% grade). Blood (baseline, pre-exercise, 4, 24, 48, and 72 h post-exercise) was diluted (1:10) and combined with LPS (20 µg/mL) for 24 h. Total RNA was isolated from leukocytes and analyzed for 574 immune-associated mRNA (Nanostring nCounter; ROSALIND.BIO). Data were expressed as log2 fold change from baseline for each condition (MSM and placebo). Compared to placebo, MSM supplementation was associated with an improved inflammation response (15 mRNA) and viral immune response (2 mRNA). The largest number of changes were found at 4 and 24 h post-exercise. The key finding in the present study is that MSM supplementation can improve inflammation management and the innate immune response after exercise.

查看原文本刊更多论文

补充MSM与人类在一轮下坡跑步后体外lps刺激后炎症减少和先天免疫反应改善有关

运动引起的肌肉损伤和随后释放的损伤相关分子模式(DAMP)导致疼痛和炎症。膳食补充剂可以通过支持炎症的消退来加速恢复的速度。本研究的目的是确定甲基磺酰甲烷(MSM)补充(运动前30天和恢复期间)是否会改变一轮下坡跑步后暴露于lps的血液白细胞的mRNA表达。运动包括60分钟的下坡跑(- 15%坡度)。将血液(基线、运动前、运动后4、24、48和72 h)稀释(1:10)，并与LPS(20µg/mL)联合24小时。从白细胞中分离总RNA，分析574种免疫相关mRNA (Nanostring nCounter;ROSALIND.BIO)。数据表示为每一种情况(MSM和安慰剂)与基线相比的log2倍变化。与安慰剂相比，MSM补充剂与炎症反应(15mrna)和病毒免疫反应(2mrna)的改善相关。运动后4小时和24小时变化最大。本研究的关键发现是MSM补充剂可以改善运动后的炎症管理和先天免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

MLTJ-Muscles Ligaments and Tendons Journal ORTHOPEDICS-

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： MLTJ (Muscle, Ligaments and Tendons Journal) is an open access, peer-reviewed online journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal, ligament, tendon, public health, exercise physiology and kinesiology issues. Muscle, Ligaments and Tendons Journal (MLTJ) provides the platform for exchange of new clinical and scientific information in the most precise and expeditious way to achieve timely dissemination of information and cross-fertilization of ideas. It is the official journal of the Italian Society of Muscles, Ligaments and Tendons (I.S.Mu.L.T.), Società Italiana Terapia con Onde D’urto (S.I.T.O.D.) and Società Italiana Studio Piede e Caviglia (S.I.S.P.E.C)