Antibacterial and Hemolytic Activity of the Coelomic Fluid of Dendrobaena veneta (Oligochaeta, Lumbricidae) Living in Different Localities

E. Ö. A. Aydoğdu, A. Çotuk
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引用次数: 12

Abstract

Earthworms are able to protect themselves against invading microorganisms through their immune systems. Although there are many studies about defense systems of Dendrobaena veneta , this is the first report focusing on the immune properties of earthworms belonging to the same species, which live in different localities. Thus, it was aimed to compare antibacterial and hemolytic activities of earthworms collected from different localities of Istanbul (Vezneciler, Suleymaniye and Beykoz). Both activities were studied with dilution and agar diffusion techniques. In antibacterial activity assays with dilution technique, it was observed that all suspensions of coelomic fluid of D. veneta living in Beykoz had significantly strong activity, while no results could be determined in diffusion assays. Nevertheless, in dilution assays for determination of hemolytic activity, coelomic fluid of earthworms living in Suleymaniye was the most effective group. In dilution assays, hemolytic activity were in the order of (S) > (V) > (B) for human erythrocytes. As a conclusion, coelomic fluid of Beykoz earthworms was a potential agent, which can be used as an alternative drug, since this coelomic fluid was effective against bacteria but not on erythrocytes.
不同产地绿竹藻体腔液的抗菌和溶血活性
蚯蚓能够通过免疫系统保护自己免受入侵微生物的侵害。虽然有很多关于石斛baena veneta防御系统的研究,但这是第一次报道属于同一物种的蚯蚓,它们生活在不同的地方。因此,本研究旨在比较伊斯坦布尔不同地区(Vezneciler、Suleymaniye和Beykoz)蚯蚓的抗菌和溶血活性。用稀释和琼脂扩散技术研究了这两种活性。稀释法抗菌活性测定发现,在Beykoz生活的D. veneta体腔液中,所有悬浮液的抑菌活性都很强,而扩散法没有结果。然而,在测定溶血活性的稀释试验中,生活在Suleymaniye的蚯蚓体腔液是最有效的组。在稀释试验中,人红细胞的溶血活性顺序为(S) > (V) > (B)。综上所述,贝氏蚯蚓体腔液对细菌有效,对红细胞无效,是一种潜在的药物,可作为替代药物使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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