BACTERIOPHAGE GENOME AND PROTEOME STUDY OF PHYTOPATHOGENIC PSEUDOMONAS SYRINGAE BACTERIA

Abstract

The article describes results of the study of genomic and proteomic characteristics of the production-promising Ps.s-27 bacteriophage of the Ural State Agrarian University, specific for phytopathogenic Pseudomonas syringae bacteria. The sequencing results allowed to determine the DNA size of the studied bacteriophage, which amounted to 24690 bp. These data correspond to the results obtained during electrophoresis (relative error δ= δ= 0.0383). Analysis of correspondence of sequence data to annotated proteomes in GeneMark Prokaryotic V 3.26. identified 28 open reading frames. The localization analysis of the studied proteins was carried out and it was determined that capsid proteins accounted for 61.2% for Ps.s-27 of UlGAU bacteriophage. Data which characterize 28 proteins of the studied bacteriophage were systematized, these data generalize information on the number of amino acids, molecular weight, isoelectric point and gross formula. When performing analysis for Ps.s-27 UlGAU, three dimers were revealed (gene_3, gene_17, and gene_23) with molecular weights of 45.0, 49.1, and 40.9 kDa, which may indicate their potential role in the capsid structure of the studied phage. The obtained data allow us to continue compiling a passport of biological properties of the production-promising Ps.s-27 UlGAU bacteriophage, which is a part of a biopreparation with direct effect.