Isolation and Amplification of Touch DNA from Portable Computer

Miriam Jasim Shehab, M. Sabbah, D. S. Namaa, Sura Nabeel
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引用次数: 0

Abstract

    Analysis of touched DNA from crime scenes is fundamental in forensic DNA laboratories. Many factors affect the recovery of DNA from touched surfaces and then affect the quality of the final results.  The aim of this work is studying the possibility of recovery suitable amount of DNA from ltouched portable computer. The computer was cleaned with 10% Bleach then touch and DNA collected for extraction by Organic method and two STR regions D5S818 (115-163bp) and FGA (308-464bp) were amplified. The results showed that it is possible to isolate a proper amount of DNA from touched portable computer where it was amplified and then analyzed by Agarose gel electrophoresis. The conclusion is that portable computer is suitable source for forensic analysis.
便携式计算机触摸DNA的分离与扩增
对犯罪现场的接触DNA进行分析是法医DNA实验室的基础。许多因素影响DNA从接触表面的恢复,进而影响最终结果的质量。本工作的目的是研究从便携式电脑中回收适量DNA的可能性。用10%漂白剂清洗计算机,然后触摸,用有机法提取DNA,扩增两个STR区域D5S818 (115-163bp)和FGA (308-464bp)。结果表明,可以从便携式计算机上分离出适量的DNA,并对其进行扩增,然后用琼脂糖凝胶电泳分析。结论是便携式计算机是法医分析的合适来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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