Isolating and Denitrification Activity of the Dominant Bacterium in Chaohu Wetland

Abstract

This passage took soil samples from Chaohu wetland at 1.5 m buried depth. Then isolated the dominant bacteria and studied its denitrification activity under the condition of different carbon sources (methanol, ethanol and acetic acid) and C/N (3:1, 5:1, 10:1). The results suggested: (1) the nitrate removal rate of isolated strain reached 90% (named as D9); (2) under the condition of C:N was 1:1, acetic acid was the best carbon source; (3) taking acetic acid as the sole carbon source, the best C:N was 3:1. These results can provide the base for the restoration of nitrate pollution in groundwater and the prevention of Lake Eutrophication.