Therapeutic Alliance: Using N-(2,3,4,5,6-Pentahydroxylhex-1-yl)-NDithiocarbamate-L-Isoleucine Disodium to Improve the Toxicity and Survival of Cisplatin Receiving Mice

Abstract

To reduce the toxicity of cisplatin N-(2,3,4,5,6-pentahydroxylhex-1-yl)-N-dithiocarbamate-L-isoleucine diso- dium (GID) based therapeutic alliance is investigated. For the proliferation of HepG2, Hela, MES-SA, HL60 and H1299 cells, 27μM of GID based therapeutic alliance gave comparable inhibition to cisplatin alone. For implanted tumor prolif- eration in mice, 1.667μmol/kg of GID based therapeutic alliance gave higher inhibition than cisplatin alone. For cisplatin receiving mice, this therapeutic alliance effectively reduces the platinum accumulations in the organs but does not affect the platinum level in the tumor tissue. Comparing to cisplatin alone, this therapeutic alliance not only increases urea and fecal platinum levels but also increases urea excretion. All the observations imply that GID based therapeutic alliance is capable of reducing the toxicity and supporting the anti-tumor potency of cisplatin.