Is the Betalactoglobulin- and Ovalbumin-Induced In Vitro Production of Interferon-Gamma and Interleukin-4 Predictive for Atopy in the Neonate? A Prospective Cohort Study and a Review of the Literature

Pediatric asthma, allergy & immunology Pub Date : 2003-06-01 DOI:10.1089/088318703765068677

S. Sopo, G. Federico, G. Zorzi, A. Pugliese, C. Fadda, A. Stabile

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Abstract

Some studies suggest that a reduction of interferon-gamma (IFNγ), in vitro production by cord blood mononuclear cells (CBMC) could be considered as a marker of atopic predisposition. The aim of our study is to estimate the usefulness of testing interleukin-4 (IL4) and IFNγ in CBMC culture supernatants after stimulus with betalactoglobulin (BLG) and ovalbumin (OVA) in predicting atopic disease. The possible correlation between the in vitro production of IL4 and IFNγ and cord blood IgE levels was also investigated. Our sample consisted of 68 neonates; among them, 28 had no atopic family history (group 0), 21 had only one parent suffering from an atopic disease (group 1), and 19 had both parents (group 2). CBMC were stimulated in vitro by 100 μg of betalactoglobulin (BLG) or ovalbumin (OVA) for 24 or 48 h. IL4 and IFNγ in the culture supernatants and cord blood IgE levels were quantified by commercial kits. Detectable production of IL4, undetectable production of IFNγ , and cord blood IgE levels of ≥0.7 kU/L...

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β -乳球蛋白和卵清蛋白诱导的干扰素- γ和白细胞介素-4的体外生成是否预示着新生儿的特应性?前瞻性队列研究及文献综述

一些研究表明，脐带血单核细胞(CBMC)体外产生干扰素γ (IFNγ)的减少可能被认为是特应性易感性的标志。本研究的目的是评估β -乳球蛋白(BLG)和卵清蛋白(OVA)刺激后CBMC培养上清中检测白细胞介素-4 (IL4)和IFNγ在预测特应性疾病中的作用。我们还研究了体外il - 4和ifn - γ的产生与脐带血IgE水平之间可能的相关性。我们的样本包括68名新生儿;其中28例无特应性家族史(0组)，21例父母中有一人患有特应性疾病(1组)，19例父母双方均患有特应性疾病(2组)。体外用100 μg的β -乳球蛋白(BLG)或卵清蛋白(OVA)刺激CBMC 24或48 h，用商业试剂盒测定培养上清液中IL4和IFNγ及脐带血IgE水平。il - 4产生可检测，ifn - γ产生不可检测，脐带血IgE水平≥0.7 kU/L…

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Pediatric asthma, allergy & immunology

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