Evaluation of Heavy Metals Resistant Micrococcus sp. Isolated from Rivers in Basra, Iraq

Journal of Bioremediation and Biodegradation Pub Date : 2017-01-31 DOI:10.4172/2155-6199.1000383

M. Anwar, S. Ali, A. Asaad

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引用次数: 3

Abstract

The present study aims to isolation, identification and characterization of heavy metal resistant bacteria from six rivers in Basra Southern Iraq. Two species of Micrococcus (M. halobius and M. kristinae) were isolated on the basis of morphological and biochemical profiles and selected based on high levels of heavy metals (cadmium, lead, copper and nickel) resistance. The concentrations of dissolved heavy metals (Cd, Pb, Cu and Ni) in rivers were determined. The maximum tolerance concentration (MTC) of isolates against Pb, Cu, Cd and Ni was determined in solid media after 72 h incubation. All isolates were resistant to Pb (2200-2600) μg mL-1, Cu (400) μg mL-1, Cd (300-400) μg mL-1 and Ni (200-300) μg ml-1. Living biomass of M. halobius and M. kristinae were used for the removal of heavy metal ions at different concentrations (25, 50 and 100) μg mL-1 from aqueous metal solutions. The best concentration of removal was 25 μg mL-1 after 72 h incubation at 120 rpm. Percent removal efficiency of M. kristinae was 47.22, 26.26 for Cu and Pb respectively, and for M. halobius was 31.52, 27.87 for Cd and Ni respectively.

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伊拉克巴士拉河中重金属耐药微球菌的鉴定

本研究旨在从伊拉克南部巴士拉的6条河流中分离、鉴定和表征重金属抗性细菌。根据形态和生化特征分离出两种微球菌(M. halobius和M. kristinae)，并根据其对重金属(镉、铅、铜和镍)的高抗性进行选择。测定了河流中溶解重金属Cd、Pb、Cu和Ni的浓度。在固体培养基中培养72 h，测定菌株对Pb、Cu、Cd和Ni的最大耐受浓度。所有菌株对Pb(2200 ~ 2600)、Cu(400)、Cd(300 ~ 400)、Ni(200 ~ 300)均具有抗性。利用M. halobius和M. kristina的活生物量去除金属水溶液中不同浓度(25、50和100)μ mL-1的重金属离子。120 rpm孵育72 h后，最佳去除浓度为25 μg mL-1。kristinae对Cu、Pb的去除率分别为47.22%、26.26%，halobius对Cd、Ni的去除率分别为31.52%、27.87%。

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Journal of Bioremediation and Biodegradation

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