Approbation of the method of enzymatic hydrolysis on natural and dyed hair for the extraction of medicinal substances

Abstract

The objective of the present study was to test the developed methods of enzymatic hydrolysis on natural and dyed fur (hair) and analyze the effect of bleaching on the results of a chemical toxicological analysis. The experiments were carried out with the used laboratory animals (red hair and black hair female guinea pigs) that had been daily given a diphenhydramine solution per os during 6 months preceding the study. It was shown that natural black and red hair (fur) have a similar composition of endogenous substances. Bleaching is a process of gaining much lighter hair color than natural one. And along with this, there is a destruction of natural hair pigment. The effect of the bleaching does not affect the background level of endogenous substances and does not interfere with the further determination of the model drug substance. Methods of enzymatic hydrolysis can be used to isolate substances from both natural colored and dyed hair (fur). The degree of extraction of diphenhydramine, a model drug substance, from bleached fur was 1.5-2 times higher than the degree of extraction of it from natural colored, which should be take into consideration when interpreting the results. This study can be used to diagnose the use of narcotic drugs and psychotropic substances. It is planned to conduct further studies on sectional analysis of hair. of synthetic psychoactive substances and their metabolites in and nails by liquid chromatography methods with mass spec-trometric detection] Informacionnoe Стрелова О.Ю., Сивакова М.А. 2017. Применение методики