Biological Validation of a Chemical Effluent Decontamination System.

IF 0.5 Q4 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Applied Biosafety Pub Date : 2020-07-09 DOI:10.1177/1535676020937967

C. Cote, J. Weidner, C. Klimko, Ashley E. Piper, Jeremy A Miller, M. Hunter, J. Shoe, J. Hoover, Brian R. Sauerbry, T. Buhr, J. Bozue, David E. Harbourt, Pamela J. Glass

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引用次数: 0

Abstract

Introduction: Failure of an existing effluent decontamination system (EDS) prompted the consideration of commercial off-the-shelf solutions for decontamination of containment laboratory waste. A bleach-based chemical EDS was purchased to serve as an interim solution. Methods: Studies were conducted in the laboratory to validate inactivation of Bacillus spores with bleach in complex matrices containing organic simulants including fetal bovine serum, humic acid, and animal room sanitation effluent. Results: These studies demonstrated effective decontamination of >106 spores at a free chlorine concentration of ≥5700 parts per million with a 2-hour contact time. Translation of these results to biological validation of the bleach-based chemical EDS required some modifications to the system and its operation. Discussion: The chemical EDS was validated for the treatment of biosafety levels 3 and 4 waste effluent using laboratory-prepared spore packets along with commercial biological indicators; however, several issues and lessons learned identified during the process of onboarding are also discussed, including bleach product source, method of validation, dechlorination, and treated waste disposal.

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化学污水净化系统的生物验证。

简介:现有的污水净化系统(EDS)的失败促使考虑商业现成的解决方案，以净化容器实验室废物。购买了一种基于漂白剂的化学物质EDS作为临时解决方案。方法:在实验室进行研究，验证在含有有机模拟物(包括胎牛血清、腐植酸和动物房间卫生污水)的复杂基质中使用漂白剂灭活芽孢杆菌孢子。结果:这些研究表明，游离氯浓度≥5700 ppm，接触时间为2小时，可有效去污>106个孢子。将这些结果转化为基于漂白剂的化学EDS的生物学验证需要对系统及其操作进行一些修改。讨论:利用实验室制备的孢子包和商业生物指示剂，验证了化学EDS处理生物安全等级3和4的废水;然而，也讨论了在入职过程中确定的几个问题和经验教训，包括漂白剂产品来源、验证方法、脱氯和处理后的废物处置。

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来源期刊

Applied Biosafety Environmental Science-Management, Monitoring, Policy and Law

CiteScore

2.50

自引率

13.30%

发文量

期刊介绍： Applied Biosafety (APB), sponsored by ABSA International, is a peer-reviewed, scientific journal committed to promoting global biosafety awareness and best practices to prevent occupational exposures and adverse environmental impacts related to biohazardous releases. APB provides a forum for exchanging sound biosafety and biosecurity initiatives by publishing original articles, review articles, letters to the editors, commentaries, and brief reviews. APB informs scientists, safety professionals, policymakers, engineers, architects, and governmental organizations. The journal is committed to publishing on topics significant in well-resourced countries as well as information relevant to underserved regions, engaging and cultivating the development of biosafety professionals globally.