Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Ulrike Schweigel, Petros Batsios, A. Müller-Taubenberger, R. Gräf, Marianne Grafe
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引用次数: 1

Abstract

ABSTRACT Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.
盘基ostellum spastin参与了半封闭有丝分裂过程中的核膜动力学
变形虫盘基ostelium amoebae进行半闭式有丝分裂,在核分裂过程中,核膜在有丝分裂中心体的插入位置和中央纺锤体周围开孔。在末期,中心体迁移到细胞核的细胞质一侧,中央纺锤体解体,核孔关闭。我们的数据表明,Dictyostelium spastin (DdSpastin)是一种微管结合和切断I型膜蛋白,在这一过程中起作用。它的有丝分裂定位符合去除微管的要求,因为微管会阻碍窗口的关闭。此外,在BioID分析中,DdSpastin与HeH/ lem家族蛋白Src1以及核膜蛋白Sun1相互作用,并与Src1、Sun1、ESCRT组分CHMP7和ist1样蛋白丝动蛋白共定位,表明动物和变形虫盘形骨之间有丝分裂核膜重塑的主要途径是保守的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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