Content Determination of Vitamin C in Durian Endocarp and Optimization of Extraction Process

Abstract

Purpose: To optimize the extraction process of vitamin C from durian endo-carp, and to determine the content of vitamin C in durian endocarp with high performance liquid chromatography (HPLC). Method: Optimize ultrasonic extraction conditions by L9 (4 3 ) orthogonal experiment by using octadecylsi-lane chemically bonded silica as the stationary phase, using methanol (A): 0.1% oxalic acid (B) = 5:95 (V/V) as the mobile phase, setting the flow rate as 1.0 mL/min, and setting the wavelength as 254 nm for assay. Result: The optimal extraction process is as follows: the material-liquid ratio is 1:12, the extraction solvent is 2% oxalic acid aqueous solution, and the extraction time is 30 min; Vitamin C has a good linearity within the concentration range of 5.4 - 108.0 mg·mL −1 , and the regression equation is y = 37698x − 61035 (R 2 = 0.9996); the average recovery rate is 99.03%, the instruments are of high precision with good stability. Conclusion: This extraction process performs well in simplicity, costs and extraction efficiency, which may accelerate the development and utilization of vitamin C extraction from durian endocarp, and provide references to relevant studies and practices.