Chromogenic media for the detection and/or enumeration of Listeria monocytogenes - results of trials performed by a working group of the International Organization for Standardization - ISO/TC 34/SC 9

IF 0.2 4区农林科学 Q4 CHEMISTRY, APPLIED

Archiv Fur Lebensmittelhygiene Pub Date : 2007-01-01 DOI:10.2377/0003-925X-58-47

R. Beumer, W. Hazeleger

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引用次数: 4

Abstract

The solid selective media PALCAM and Oxford agar originally described in the ISO (International Organization for Standardization) Standard 11290 part 1 and part 2 "Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes", suffer from the disadvan-tage that L monocytogenes cannot be differentiated from non-pathogenic liste-rias.On the basis of the results of trials, organised by an ISO/TC 34/SC 9 working group with blood-based and chromogenic media, ISO members agreed that the ALOA medium and Rapid'L.mono agar performed significantly better than the other media tested.Moreover, the ready-to-use ALOA plates performed similarly to the medium prepared from the individual components. Finally Substitution of the expensive component phosphatidylinositol (Pl), Substrate for phospholipase C activity of L monocytogenes, by lecithin had no noticeable effect on the elective features of the ALOA medium, provided the lecithin contained a sufficiently high concentration of Pl.

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用于检测和/或枚举单核细胞增生李斯特菌的显色培养基-国际标准化组织工作组进行的试验结果- ISO/TC 34/SC 9

固体选择性培养基PALCAM和牛津琼脂最初在ISO(国际标准化组织)标准11290第1部分和第2部分“食品和动物饲料的微生物学-单细胞增生李斯特菌检测和计数的水平方法”中描述，其缺点是单细胞增生李斯特菌无法与非致病性李斯特菌区分。根据ISO/TC 34/SC 9工作小组组织的血液和显色培养基试验结果，ISO成员同意ALOA培养基和Rapid'L。单株琼脂的表现明显优于其他培养基。此外，即用型ALOA板的性能与由单个组分制备的培养基相似。最后，如果卵磷脂含有足够高浓度的磷脂酰肌醇(Pl)，则卵磷脂取代昂贵的磷脂酰肌醇(Pl)，即L单核增生细胞磷脂酶C活性的底物，对ALOA培养基的选择性特征没有明显影响。

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来源期刊

Archiv Fur Lebensmittelhygiene 工程技术-毒理学

自引率

0.00%

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审稿时长

>12 weeks

期刊介绍： The "Journal of Food Safety and Food Quality“ provides a platform for papers including case studies and discussion papers dealing with topics from all areas of food hygiene (food originating from animals) including dairy hygiene, food monitoring, beef cattle and meat examination, meat hygiene and food technology.