Cadmium induced genotoxicity and antioxidative defense system in lentil (Lens culinaris Medik.) genotype

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2023-02-12 DOI:10.36253/caryologia-1666

D. Shahwar, Z. Khan, M. Ansari

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引用次数: 1

Abstract

Induced mutagenesis is considered a coherent mechanism in crop improvement programmes to produce novel plant varieties. Due to the insufficiency of desired genotypes, plant breeders are supposed to re-associate the gene of interest from the accessible gene pool of the related plant species through hybridization to develop new cultivars with desired traits. The present investigation was performed to evaluate cadmium induced mutagenesis on growth performance, physio-biochemical traits and DNA damage studies in lentil. Growth and morphological parameters exhibited reduction with increasing concentration of cadmium. Maximum devaluation was reported at the highest concentration. Physiological and biochemical traits were also affected by different cadmium concentrations and reduced as concentration increased. Lipid peroxidation activity and antioxidant enzymes increased as mutagenic stress increased caused by cadmium. CAT and SOD concentration was found to increase initially and then decreased gradually at higher cadmium concentrations. SEM analysis of stomatal morphology revealed variation in stomatal shape and size in treated populations. There was a gradual enhancement in the percentage of DNA damage along with variation in morphological traits. The DNA damage was recorded as precocious movement, stray bivalent, laggard, stickiness, disorientation of chromosome, multi-bridge, disturbed polarity and micronuclei. It was concluded that at higher concentrations, cadmium cause DNA damage and these chromosomal alterations causes morpho-physiological and biochemical changes in lentil.

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镉对小扁豆(Lens culinaris Medik.)基因型遗传毒性及抗氧化防御系统的影响

诱变被认为是作物改良计划中产生新植物品种的一种连贯机制。由于所需基因型的不足，植物育种者应该通过杂交从相关植物物种可获得的基因库中重新组合感兴趣的基因，以开发具有所需性状的新品种。本文研究了镉诱变对小扁豆生长性能、生理生化特性和DNA损伤的影响。随着镉浓度的增加，植株的生长和形态参数呈下降趋势。在浓度最高时，贬值幅度最大。不同镉浓度对植株的生理生化性状也有影响，且随着镉浓度的升高而降低。脂质过氧化活性和抗氧化酶随镉致突变胁迫的增加而升高。在较高的镉浓度下，CAT和SOD浓度先升高后逐渐降低。气孔形态的扫描电镜分析揭示了处理群体气孔形状和大小的变化。随着形态特征的变化，DNA损伤的百分比逐渐增加。DNA损伤表现为染色体早动、杂散二价、滞后、粘滞、染色体失向、多桥、极性紊乱和微核。由此可见，在较高镉浓度下，镉对小扁豆的DNA造成损伤，这些染色体的改变引起了小扁豆形态生理生化的改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.