MOLECULAR ANALYSIS OF A PUTATIVE EN/SPM-RELATED TRANSPOSON PROTEIN IN BRASSICA JUNCEA

Abstract

Open reading frame (ORF) of Putative En/Spm transposon in Brassica potentially helps to understand the relationship between various eukaryotic transposable elements. The current study was initially conducted to isolate and analyze the putative En/Spm-related transposon gene from Brassica juncea. PCR products (750 bp) from B. juncea (accession PI 649105 and PI 271442) were cloned, sequenced, and analyzed. Results of BLAST showed identical sequences between two accessions with 100% similarity. The amplified DNA transposon and conserved domain compared to the GenBank database to evaluate the genetic diversity relationships. Sequence of this putative transposon gene from B. juncae was a 98% similar to B. rapa subsp pekinensis at the nucleotide level, and 94% with the En/Spm-related transposon protein of B. oleracea at the amino acid level. Conserved domain architecture was related to transposase_21_pfam0299 and transposase family tnp2 and had a relationship with space outside the cell structure and/or to space outside the plasma membrane.