Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption

IF 1.5 4区材料科学 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY

Micro & Nano Letters Pub Date : 2023-05-26 DOI:10.3390/micro3020038

A. Maslakova, K. Prusakov, Anastasia Sidorova, E. Pavlova, A. Ramonova, D. Bagrov

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引用次数: 0

Abstract

Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, their intricate porous structure can impede diffusion and render some analyte-binding sites inaccessible. To overcome these diffusion limitations and improve analyte adsorption onto the polymer, a pressure-driven sample flow through the membrane can be employed. To date, the efficiency of pressure-driven analyte delivery into these membranes has not been quantified. Here, we compare forced flow and passive sample diffusion through poly(dioxanone) electrospun membranes. We examine two model analytes, BSA and interleukin-1 beta (IL1b), to address both non-specific and specific binding. Following exposure of the membranes to the test solutions, we measured the residual concentrations of the analytes using fluorometry and enzyme-linked immunosorbent assay (ELISA) techniques. The pressure-driven sample loading was superior to passive diffusion, with a 2.8–11.5-fold change for physical adsorption and a 2.4–3.4-fold difference for specific binding. Our data can be useful for the development of immunoassays and microfluidic devices.

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压力驱动的样品流通过静电纺丝膜增加了分析物的吸附

电纺丝聚合物膜由于其大的比表面积和多种化学修饰的机会而被认为是有前景的生物传感器组件。然而，它们复杂的多孔结构会阻碍扩散，使一些分析物结合位点无法进入。为了克服这些扩散限制并改善分析物在聚合物上的吸附，可以采用压力驱动的样品流通过膜。到目前为止，压力驱动的分析物进入这些膜的效率还没有被量化。在这里，我们比较了强迫流动和被动样品扩散通过聚二氧环酮静电纺丝膜。我们检查了两种模型分析物，BSA和白细胞介素-1 β (il - 1b)，以解决非特异性和特异性结合。将膜暴露于测试溶液后，我们使用荧光法和酶联免疫吸附测定(ELISA)技术测量分析物的残留浓度。压力驱动的样品加载优于被动扩散，物理吸附差异2.8 - 11.5倍，特异性结合差异2.4 - 3.4倍。我们的数据可用于开发免疫测定和微流体装置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Micro & Nano Letters 工程技术-材料科学：综合

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

2.8 months

期刊介绍： Micro & Nano Letters offers express online publication of short research papers containing the latest advances in miniature and ultraminiature structures and systems. With an average of six weeks to decision, and publication online in advance of each issue, Micro & Nano Letters offers a rapid route for the international dissemination of high quality research findings from both the micro and nano communities. Scope Micro & Nano Letters offers express online publication of short research papers containing the latest advances in micro and nano-scale science, engineering and technology, with at least one dimension ranging from micrometers to nanometers. Micro & Nano Letters offers readers high-quality original research from both the micro and nano communities, and the materials and devices communities. Bridging this gap between materials science and micro and nano-scale devices, Micro & Nano Letters addresses issues in the disciplines of engineering, physical, chemical, and biological science. It places particular emphasis on cross-disciplinary activities and applications. Typical topics include: Micro and nanostructures for the device communities MEMS and NEMS Modelling, simulation and realisation of micro and nanoscale structures, devices and systems, with comparisons to experimental data Synthesis and processing Micro and nano-photonics Molecular machines, circuits and self-assembly Organic and inorganic micro and nanostructures Micro and nano-fluidics