Applications of flow cytometry for the detection and characterization of biological aerosols

Field Analytical Chemistry and Technology Pub Date : 1999-01-01 DOI:10.1002/(SICI)1520-6521(1999)3:4/5<291::AID-FACT8>3.0.CO;2-5

S. Sincock, H. Kulaga, M. Cain, Patricia R. Anderson, P. Stopa

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引用次数: 16

Abstract

Flow cytometry (FCM), unlike conventional forms of spectroscopy, allows one to interrogate individual members of a sample population for size, shape, biological, and/or chemical properties. Over the past several years, we have investigated the use of flow cytometry to detect and identify microorganisms in aerosols. The effort to date has shown that changes in scatter patterns or DNA composition suggest that changes in the ambient air are evident. The use of macromolecular dyes and antibodies tagged by fluorescence can be used to identify specific components in the aerosol. Moreover, it is possible to combine several of these modes into one step. The assays presented here take 5 min or less to perform. Strategies for the use of flow cytometry to characterize biological aerosols will be discussed. Both laboratory and field studies that demonstrate the potential use of FCM as a means to detect and characterize microbiological materials in aerosols are discussed. © 1999 John Wiley & Sons, Inc.* Field Analyt Chem Technol 3: 291–306, 1999

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流式细胞术在生物气溶胶检测和表征中的应用

与传统形式的光谱学不同，流式细胞术(FCM)允许人们询问样本群体的个体成员的大小，形状，生物和/或化学性质。在过去的几年中，我们研究了使用流式细胞术检测和鉴定气溶胶中的微生物。迄今为止的努力已经表明，散布模式或DNA组成的变化表明，环境空气的变化是明显的。使用大分子染料和荧光标记的抗体可用于鉴定气溶胶中的特定成分。此外，可以将这些模式中的几个组合到一个步骤中。这里介绍的检测需要5分钟或更短的时间来完成。将讨论使用流式细胞术表征生物气溶胶的策略。讨论了证明FCM作为气溶胶中微生物材料检测和表征手段的潜在用途的实验室和现场研究。©1999 John Wiley & Sons, Inc.* Field analyst Chem technology 3: 291 - 306,1999

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Field Analytical Chemistry and Technology

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