Improvement of sperm quality of the depik fish, rasbora tawarensis, after cryopreservation using antioxidant.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-01-01 DOI:10.54680/fr23110110312

Z. Muchlisin, D. Afriani, K. Eriani, I. Hasri, F. Nur, S. Maulida, L. Handayani, F. Kocabaş, M. S. Siti-Azizah

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引用次数: 0

Abstract

BACKGROUND The cryopreservation of the sperm of the depik fish, Rasbora tawarensis, has previously been developed. However, the quality of the sperm post cryopreservation was not satisfactory and might be improved through the application of antioxidants. OBJECTIVE To determine the most suitable antioxidant for the cryopreservation of the depik fish spermatozoa. MATERIALS AND METHODS A completely randomized design with a non-factorial experiment was used and the tested antioxidants were glutathione, beta-carotene, ascorbic acid, and butylated hydroxytoluene (BHT) at 6 % concentrations. All treatments had three replications. The sperms were collected from 10 male fishes and diluted with Ringer solution in a ratio of 1: 20 (v/v, sperm: Ringer solution). Then 5% DMSO and 5 % egg yolk were added to the diluted sperms. Furthermore, 6 % of the tested antioxidants were added to the diluents, and then, cryopreservation was carried out in liquid nitrogen for 14 days. RESULTS The ANOVA test showed that the application of antioxidants significantly affected the sperm motility, fertility, and hatching rates of the eggs (P < 0.05). Furthermore, the antioxidants also protected the sperm cells during cryopreservation, with glutathione being the best antioxidant. CONCLUSION The application of antioxidants during the cryopreservation of depik fish sperm had a significant effect on motility, fertility and hatchability of eggs post-cryo. Furthermore, glutathione was the most suitable antioxidant. doi.org/10.54680/fr23110110312.

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使用抗氧化剂对冷冻保存后的塔华鱼精子质量的改善。

之前已经开发了冷冻保存depik鱼(Rasbora tawarensis)精子的方法。但冷冻后的精子质量不理想，可以通过抗氧化剂的应用来改善。目的确定冷冻保存depik鱼精子最合适的抗氧化剂。材料与方法采用完全随机设计，无因子试验，抗氧化剂为谷胱甘肽、β -胡萝卜素、抗坏血酸和6%浓度的丁基羟基甲苯(BHT)。所有的治疗都有三个重复。采集10只雄鱼的精子，用林格液按1:20的比例(v/v，精子:林格液)稀释。然后在稀释后的精子中加入5%的DMSO和5%的蛋黄。在稀释剂中加入6%的抗氧化剂，在液氮中低温保存14天。结果方差分析显示，抗氧化剂的使用显著影响了精子活力、受精率和卵子的孵化率(P < 0.05)。此外，抗氧化剂对精子冷冻保存也有保护作用，其中谷胱甘肽是最好的抗氧化剂。结论抗氧化剂在depik鱼精子冷冻保存过程中的应用对卵子冷冻后的活力、生育能力和孵化能力有显著影响。谷胱甘肽是最合适的抗氧化剂。doi.org/10.54680/fr23110110312。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.