Gigantic optical non-linearities from nanoparticle-enhanced molecular probes with potential for selectively imaging the structure and physiology of nanometric regions in cellular systems

Gadi Peleg, Aaron Lewis, Oleg Bouevitch, Leslie Loew, Dorit Parnas, Michal Linial
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引用次数: 26

Abstract

The requirement to functionally probe biological structures, with ever increasing selectivity and three-dimensional resolution is a frontier area in microscopy. Non-linear optics has a unique potential in this regard with numerous studies focused on the potential of three-dimensional imaging with super-resolution. In this paper we demonstrate that non-linear optical phenomena, such as second harmonic (SH) generation, which is very sensitive to the membrane potential, can be locally enhanced by complexing or approaching a SH generating molecular probe to a nanoantenna of a silver or gold nanoparticle. This gives complexes with gigantic optical non-linearities. These contrast enhancing non-linear optical complexes have the potential to be directed selectively to specific nanometric regions in cells in order to report on alterations on the structure and the function in such regions while overcoming the inherent inefficiency of non-linear optical interactions.

纳米颗粒增强分子探针的巨大光学非线性,具有选择性成像细胞系统中纳米区域结构和生理学的潜力
以不断提高的选择性和三维分辨率对生物结构进行功能性探测的要求是显微镜学的前沿领域。非线性光学在这方面具有独特的潜力,许多研究都集中在超分辨率三维成像的潜力上。在本文中,我们证明了非线性光学现象,如对膜电位非常敏感的二次谐波(SH)产生,可以通过将产生SH的分子探针络合或接近银或金纳米颗粒的纳米天线来局部增强。这就产生了具有巨大光学非线性的配合物。这些增强对比度的非线性光学复合物有可能选择性地指向细胞中的特定纳米区域,以便报告这些区域中结构和功能的变化,同时克服非线性光学相互作用的固有低效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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