J D Bhawalkar, A Shih, S J Pan, W S Liou, J Swiatkiewicz, B A Reinhardt, P N Prasad, P C Cheng
{"title":"Two-photon laser scanning fluorescence microscopy-from a fluorophore and specimen perspective","authors":"J D Bhawalkar, A Shih, S J Pan, W S Liou, J Swiatkiewicz, B A Reinhardt, P N Prasad, P C Cheng","doi":"10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8","DOIUrl":null,"url":null,"abstract":"<p>Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 3","pages":"168-178"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199609)4:3<168::AID-BIO7>3.0.CO;2-8","citationCount":"25","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199609%294%3A3%3C168%3A%3AAID-BIO7%3E3.0.CO%3B2-8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 25
Abstract
Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
