{"title":"Effect of the detector size and the fluorescence wavelength on the resolution of three- and two-photon confocal microscopy","authors":"Min Gu, X S Gan","doi":"10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","DOIUrl":null,"url":null,"abstract":"<p>Image formation in three- and two-photon confocal fluorescence scanning microscopy is analysed under the paraxial approximation. The analysis incorporates the effect of a finite-sized detector and the effect of the fluorescence wavelength. The resolution in the transverse and axial directions is examined for edge and layer objects, respectively. For a given excitation energy gap, three-photon confocal and non-confocal fluorescence microscopes feature a resolution close to that of their two-photon counterparts. However, the resolution of the three-photon microscopes is improved by up to 40-50% when the same excitation wavelength is used. This may prove advantageous in practical three-photon fluorescence microscopy.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 3","pages":"129-137"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199609%294%3A3%3C129%3A%3AAID-BIO3%3E3.0.CO%3B2-L","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 13
Abstract
Image formation in three- and two-photon confocal fluorescence scanning microscopy is analysed under the paraxial approximation. The analysis incorporates the effect of a finite-sized detector and the effect of the fluorescence wavelength. The resolution in the transverse and axial directions is examined for edge and layer objects, respectively. For a given excitation energy gap, three-photon confocal and non-confocal fluorescence microscopes feature a resolution close to that of their two-photon counterparts. However, the resolution of the three-photon microscopes is improved by up to 40-50% when the same excitation wavelength is used. This may prove advantageous in practical three-photon fluorescence microscopy.
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