Effetti della low-level laser irradiation su cellule staminali mesenchimali inoculate in una biomatrice tridimensionale: studio pilota in vitro

Abstract

Objectives

The purpose of this trial was to evaluate the effects of Nd:Yag laser irradiation on proliferation and differentiation of mesenchymal stem cells (MSCs) induced to differentiate into osteoblasts.

Materials and methods

MSCs were isolated from adult human bone marrow and cultured in complete medium (α-MEM). The cells were subsequently cultured in osteogenic medium, seeded into three-dimensional collagen scaffolds, and incubated. We used six scaffolds, equally divided into three groups: two groups were irradiated with Nd:Yag laser at different power levels, and the third was left untreated (control group). Cells were specifically stained and assessed at 7 and 14 days.

Results

After 7 days, proliferation in the laser-treated scaffolds was increased with respect to the control scaffold. After 14 days, however, laser irradiation does not appear to have any further effect on cell proliferation. As for differentiation, an exponential increase (versus controls) was observed after 14 days of laser irradiation.

Conclusions

Laser treatment clearly exerts positive effects on the proliferation and differentiation of MSCs induced to differentiate into cells of the osteoblastic lineage.