A model for anticancer surveillance was pharmacologically developed to evaluate vitality principle in breast cancer rats

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Liu Jinyu , Zhang Mengyang , Zhao Xin , Ge Shasha , Li Shuang , Peng Lin , Mu Yuxue , Chen Chen , Li Xiaoya , Zhang Rui , Feng Xuanye , Deng Bo , Jia Liqun , Lin Yulin , Wang Yueqi , Cheng Zhiqiang , TaeHoo Yi , Cai Dayong
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引用次数: 0

Abstract

OBJECTIVE

To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.

METHODS

The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene (DMBA, i.g., 100 mg/kg) at d001. The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant (CFA), the various intervals (k = 0.80) were dominated from d025 (600.00 h) to d095 (2288.82 h). The optimal surveillant status was confirmed with the median effective interval (EI50) from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis. The availability of this model was confirmed with Shugan Liangxue prescription (SLP), from the vitality principle, and assured further from interleukin-12 levels.

RESULTS

The regressive curve was set up between the intervals and tumor volumes, the EI50 in SLP-treated rats (1475.00 h, YSLP = 0.1026 + 0.8780/[1 + 10(27.1425-8.565×)]) was postponed, which was 1.87 multiple of the EI50 in CFA rats (791.40 h, y = −0.0525 + 0.9452/[1 + 10(30.4870-10.52×)], so did prepone the curve between the intervals and the immunological biomarker, serum inter-leukin-12 levels, the EI50 in SLP-treated rats (744.90 h, YSLP = −0.0145 + 0.7455/[1 + 10(52.09636-18.13×)]) be 0.78 multiple of the EI50 in CFA rats (960.10 h, YCFA = 0.2460 + 0.7270/[1 + 10(− 67.1546 + 22.52×)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.

CONCLUSION

The model for anticancer surveillance was pharmacologically established as the optimal interval (791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available model was confirmed with SLP, from the vitality principle, for evaluating immunological effects against breast cancer.

建立了癌症大鼠抗癌药物监测模型,评价其活力原理
目的建立癌症大鼠抗癌监测模型,评价其活力原理。方法用7,12-二甲基苯[a]蒽(DMBA,i.g.,100mg/kg)在d001复制大鼠癌症。抗癌监测被定义为初次致敏和用完全弗氏佐剂(CFA)搅拌的第一次激发之间的间隔,不同的间隔(k=0.80)主要是从d025(600.00小时)到d095(2288.82小时)。用肿瘤体积回归曲线的中位有效区间(EI50)确定最佳监测状态,用于建立药效学模型。用肿瘤和肿瘤浸润性淋巴细胞组织病理学方法证实CFA对乳腺癌症肿瘤发生的免疫监测作用。舒肝凉血方(SLP)从活力原理证实了该模型的可用性,并从白细胞介素-12水平进一步保证了该模型。结果建立了间隔与肿瘤体积的回归曲线,SLP处理大鼠的EI50(1475.00h,YSLP=0.1026+0.8780/[1+10(27.1425-8.565×,血清白细胞介素-12水平,SLP治疗大鼠的EI50(744.90 h,YSLP=-0.0145+0.7455/[1+10(52.09636-18.13×)])是CFA大鼠的EI50(960.10 h,YCFA=0.246+0.77270/[1+10)的0.78倍,这种免疫作用介导了抗癌预后。肿瘤组织学证实,具有CFA搅拌免疫的SLP大鼠中激活的肿瘤浸润淋巴细胞比仅接受CFA.结论抗癌监测模型在药理学上被建立为初次致敏和用完全弗氏佐剂搅拌的第一次激发之间的最佳间隔(791.40 h)。从活力原理出发,用SLP证实了这种可用的模型,用于评估对癌症的免疫效果。
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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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