Le spermatozoide de Ophidion sp. (Poisson, téléostéen): Particularités ultrastructurales du flagelle

Abstract

The spermatozoon of Ophidion sp. possesses an elongated nucleus 8 μm long, a short midpiece (0,6 μm), and a long flagellum (100 μm). The flagellar membrane extends in the form of two diametrically opposed sidefins. Evolving spermatids and spermatozoa are found in the lumen of the seminiferous tubes. The sections of flagella show filamentary and tubular elements disposed parallel to the axoneme microtubules. We have divided the flagella into three types. In type 1 the tip of the sidefins contains 20 to 30 filaments 5 run in diameter and between these and the axoneme 20 to 30 tubular elements 15 to 20 nm in diameter. Type 2 possesses a dense cytoplasm and a few tubular elements 10 nm in diameter disposed at the tip of the sidefins. Type 3 contains a cytoplasm which is not dense and in which we found polysaccharides and 1 to 8 tubular elements forming a palisade which lines the plasma membrane at the tip of the sidefins. We interpret these three types as three successive stages in the organization of the flagellum during spermiogenesis. Type 3 corresponds to the spermatic flagellum. These 10-nm-diameter tubules do not have the same chemical composition as the microtubules. Elements of the cytoskeleton serve as a support for the sidefins.