{"title":"Quantitative Analysis of Changes in the Molecular Species of Glycerolipids in Cultured Cells during Signal Transduction","authors":"Lee Chunghee, Hajra Amiya K.","doi":"10.1006/ncmn.1993.1041","DOIUrl":null,"url":null,"abstract":"<div><p>A method for the quantitative analysis of the molecular species of glycerolipids present In biological samples has been described. 1,2-Diacyl-<em>sn</em>-glycerol, either isolated from biological samples or enzymatically generated from phosphoglycerides, is benzoylated at the <em>sn</em>-3 position and then subjected to reverse-phase (C<sub>18</sub>-silica) HPLC to separate the molecular species of different hydrophobicitles. An internal standard (1,2-distearoyl-<em>sn</em>-glycerol) is used to identify and quantify the various species eluted from the reverse-phase column. Examples are given for the quantitative analysis of molecular species and precursor-product relationships of glycerolipids generated In SK-N-SH neuroblastoma cells after stimulation of the cell-surface muscarinic acetylcholine receptors.</p></div>","PeriodicalId":100951,"journal":{"name":"Neuroprotocols","volume":"3 2","pages":"Pages 83-90"},"PeriodicalIF":0.0000,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/ncmn.1993.1041","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroprotocols","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1058674183710414","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A method for the quantitative analysis of the molecular species of glycerolipids present In biological samples has been described. 1,2-Diacyl-sn-glycerol, either isolated from biological samples or enzymatically generated from phosphoglycerides, is benzoylated at the sn-3 position and then subjected to reverse-phase (C18-silica) HPLC to separate the molecular species of different hydrophobicitles. An internal standard (1,2-distearoyl-sn-glycerol) is used to identify and quantify the various species eluted from the reverse-phase column. Examples are given for the quantitative analysis of molecular species and precursor-product relationships of glycerolipids generated In SK-N-SH neuroblastoma cells after stimulation of the cell-surface muscarinic acetylcholine receptors.
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