Efficacy of calcium hydroxide-loaded poly(lactic-co-glycolic acid) biodegradable nanoparticles as an intracanal medicament against endodontopathogenic microorganisms in a multi-species biofilm model

IF 1.3 4区医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE

Australian Endodontic Journal Pub Date : 2023-11-10 DOI:10.1111/aej.12812

Warat Leelapornpisid DDS, Piyaporn Wanwatanakul DDS, Thanisorn Mahatnirunkul BSc, MSc, PhD

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Abstract

This study aimed to evaluate the antimicrobial activity of calcium hydroxide-loaded poly(lactic-co-glycolic acid) nanoparticles (CH-loaded PLGA NPs) on multi-species biofilms. Human root blocks were prepared (n = 40), and multi-species suspensions of Candida albicans, Enterococcus faecalis and Streptococcus gordonii were incubated within the root canals for 21 days. Canals (n = 10/group) were then medicated with saline solution (negative control), chlorhexidine (positive control), calcium hydroxide and CH-loaded PLGA NPs for 7 days. Samples taken from the 0.1 mm root canal dentin were collected, and cell growth was detected by culture on BHI agar. The viable cell count of the Ca(OH)₂, chlorhexidine gel and CH-loaded PLGA NPs group was significantly lower than the normal saline group (p < 0.001). CH-loaded PLGA NPs demonstrated a significant lower viable cell than Ca(OH)₂ (p < 0.001); it has potential as a medicament for endodontic therapy.

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在多物种生物膜模型中，负载氢氧化钙的聚（乳酸-乙醇酸）可生物降解纳米颗粒作为管内药物对抗内胚层致病微生物的功效。

本研究旨在评估氢氧化钙负载的聚乳酸-羟基乙酸纳米颗粒（CH负载的PLGA NPs）对多物种生物膜的抗菌活性。制备人根块（n = 40），白色念珠菌、粪肠球菌和戈登链球菌的多物种悬浮液在根管内孵育21 天。渠道（n = 10/组）用盐水溶液（阴性对照）、氯己定（阳性对照）、氢氧化钙和CH负载的PLGA NP给药7 天。从0.1 mm根管牙本质，并通过在BHI琼脂上培养检测细胞生长。Ca（OH）2、氯己定凝胶和CH负载的PLGA NP组的活细胞计数显著低于生理盐水组（p 2（p

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来源期刊

Australian Endodontic Journal DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.50

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： The Australian Endodontic Journal provides a forum for communication in the different fields that encompass endodontics for all specialists and dentists with an interest in the morphology, physiology, and pathology of the human tooth, in particular the dental pulp, root and peri-radicular tissues. The Journal features regular clinical updates, research reports and case reports from authors worldwide, and also publishes meeting abstracts, society news and historical endodontic glimpses. The Australian Endodontic Journal is a publication for dentists in general and specialist practice devoted solely to endodontics. It aims to promote communication in the different fields that encompass endodontics for those dentists who have a special interest in endodontics.