{"title":"Sex-Specific Variations in the mRNA Levels of Candidate Genes in Peripheral Blood Mononuclear Cells from Patients with Diabetes: A Multistep Study.","authors":"Yuzhen Cen, Dana Feng, Rasoul Kowsar, Zhen Cheng, Yu Luo, Qingyu Xiao","doi":"10.1080/07435800.2023.2280571","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes (T2D) is one of the most prevalent diseases that also show sexual dimorphism in many different aspects.</p><p><strong>Objectives: </strong>This study aimed to distinguish the mRNA expression of genes in peripheral blood mononuclear cells (PBMCs) in men or women with T2D using a multistep analysis.</p><p><strong>Methods: </strong>A total of 95 patients with T2D were compared based on their sex in terms of clinical variables and mRNA expression in their PBMCs.</p><p><strong>Results: </strong>Men with T2D had lower LDLC, HDLC, and HbA1c values in their blood, but greater creatinine levels. In men with T2D, <i>TLR4, CCR2, NOX2</i>, and <i>p67phox</i> mRNA expression was greater, but <i>IL6</i> and <i>NF-κB</i> mRNA expression was lesser in PBMCs. There was a link between fasting plasma glucose (FPG), triglycerides, and hs-CRP, as well as <i>COX1</i> mRNA in men with T2D. In women with T2D, FPG was associated with the mRNA expression of <i>THBS1</i> and <i>p67phox</i>, as well as triglycerides and HDLC levels. We found the exclusive effect of FPG on HDLC, HbA1c, as well as <i>p67phox</i> mRNA in PBMCs of women with T2D. Analysis revealed the exclusive effect of FPG on hs-CRP and <i>PAFR</i> mRNA in PBMCs of men with T2D. FPG was shown to be associated with body mass index, hs-CRP, triglycerides, and <i>COX1</i> mRNA in men with T2D, and with serum triglycerides, <i>THSB1</i>, and <i>p67phox</i> mRNA in women with T2D, according to network analysis. HbA1c was linked with <i>NF-κB</i> mRNA in women with T2D.</p><p><strong>Conclusions: </strong>Using a multistep analysis, it was shown that network analysis outperformed traditional analytic techniques in identifying sex-specific alterations in mRNA gene expression in PBMCs of T2D patients. The development of sex-specific therapeutic approaches may result from an understanding of these disparities.</p>","PeriodicalId":11601,"journal":{"name":"Endocrine Research","volume":" ","pages":"59-74"},"PeriodicalIF":1.5000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/07435800.2023.2280571","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/4 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Type 2 diabetes (T2D) is one of the most prevalent diseases that also show sexual dimorphism in many different aspects.
Objectives: This study aimed to distinguish the mRNA expression of genes in peripheral blood mononuclear cells (PBMCs) in men or women with T2D using a multistep analysis.
Methods: A total of 95 patients with T2D were compared based on their sex in terms of clinical variables and mRNA expression in their PBMCs.
Results: Men with T2D had lower LDLC, HDLC, and HbA1c values in their blood, but greater creatinine levels. In men with T2D, TLR4, CCR2, NOX2, and p67phox mRNA expression was greater, but IL6 and NF-κB mRNA expression was lesser in PBMCs. There was a link between fasting plasma glucose (FPG), triglycerides, and hs-CRP, as well as COX1 mRNA in men with T2D. In women with T2D, FPG was associated with the mRNA expression of THBS1 and p67phox, as well as triglycerides and HDLC levels. We found the exclusive effect of FPG on HDLC, HbA1c, as well as p67phox mRNA in PBMCs of women with T2D. Analysis revealed the exclusive effect of FPG on hs-CRP and PAFR mRNA in PBMCs of men with T2D. FPG was shown to be associated with body mass index, hs-CRP, triglycerides, and COX1 mRNA in men with T2D, and with serum triglycerides, THSB1, and p67phox mRNA in women with T2D, according to network analysis. HbA1c was linked with NF-κB mRNA in women with T2D.
Conclusions: Using a multistep analysis, it was shown that network analysis outperformed traditional analytic techniques in identifying sex-specific alterations in mRNA gene expression in PBMCs of T2D patients. The development of sex-specific therapeutic approaches may result from an understanding of these disparities.
期刊介绍：
This journal publishes original articles relating to endocrinology in the broadest context. Subjects of interest include: receptors and mechanism of action of hormones, methodological advances in the detection and measurement of hormones; structure and chemical properties of hormones. Invitations to submit Brief Reviews are issued to specific authors by the Editors.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
