Inhibition of infantile hemangioma growth and promotion of apoptosis via VEGF/PI3K/Akt axis by 755-nm long-pulse alexandrite laser

IF 4.1 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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引用次数: 0

Abstract

Background

Infantile hemangioma (IH) is a common vascular tumor in female infants, which can lead to aesthetic issues and facial scarring. This study aimed to investigate the inhibitory effects and underlying mechanisms of 755 nm long-pulsed alexandrite laser on IH.

Methods

Hemangioma endothelial cells (HemECs) were exposed to 755 nm long-pulsed alexandrite laser to evaluate its impact on cell proliferation and apoptosis. A patient-derived xenograft model was established to assess the inhibitory effects of laser treatment on IH in vivo.

Results

In vitro, 755 nm long-pulsed alexandrite laser effectively suppressed the proliferation of HemECs and induced cell apoptosis. Laser treatment significantly inhibited the volume and weight of tumors, accompanied by significant downregulation of vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) expression levels in both hemangioma cells and tumors. Additionally, laser treatment resulted in the conversion of VEGFA165a to VEGFA165b. TUNEL staining demonstrated increased apoptosis in tumor cells after laser treatment, along with upregulation of cleaved caspase-3 and Bax, and downregulation of Bcl-2.

Conclusion

In addition to the principle of selective photothermal decomposition, modulation of the VEGF/PI3K/Akt axis may serve as a potential mechanism for IH treatment using a long pulse-width 755 nm laser. This sheds valuable light on the molecular mechanisms underlying IH pathogenesis and potential therapeutic targets while providing a theoretical basis for the safe and efficient management of proliferative IH using laser therapy.

755nm长脉冲亚历山大宝石激光通过VEGF/PI3K/Akt轴抑制婴儿血管瘤生长和促进细胞凋亡。
背景:婴儿血管瘤(IH)是女婴常见的血管瘤,可导致美观问题和面部瘢痕形成。本研究旨在探讨755nm长脉冲亚历山大宝石激光对IH的抑制作用及其潜在机制。方法:将血管瘤内皮细胞(HemECs)暴露于755nm长的脉冲亚历山大宝石激光器中,以评估其对细胞增殖和凋亡的影响。建立了患者来源的异种移植物模型,以评估激光治疗对体内IH的抑制作用。结果:755nm长脉冲紫翠宝石激光在体外能有效抑制HemECs的增殖,诱导细胞凋亡。激光治疗显著抑制肿瘤的体积和重量,同时显著下调血管瘤细胞和肿瘤中血管内皮生长因子A(VEGFA)、血管内皮生长因数受体2(VEGFR2)、磷脂酰肌醇3-激酶(PI3K)和蛋白激酶B(Akt)的表达水平。此外,激光处理导致VEGFA165a转化为VEGFA165b。TUNEL染色显示,激光治疗后肿瘤细胞凋亡增加,裂解的胱天蛋白酶-3和Bax上调,Bcl-2下调。结论:除了选择性光热分解的原理外,调节VEGF/PI3K/Akt轴可能是使用长脉宽755nm激光治疗IH的潜在机制。这为IH发病机制的分子机制和潜在的治疗靶点提供了有价值的线索,同时为使用激光治疗安全有效地管理增殖性IH提供了理论基础。
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来源期刊
Biomedical Journal
Biomedical Journal Medicine-General Medicine
CiteScore
11.60
自引率
1.80%
发文量
128
审稿时长
42 days
期刊介绍: Biomedical Journal publishes 6 peer-reviewed issues per year in all fields of clinical and biomedical sciences for an internationally diverse authorship. Unlike most open access journals, which are free to readers but not authors, Biomedical Journal does not charge for subscription, submission, processing or publication of manuscripts, nor for color reproduction of photographs. Clinical studies, accounts of clinical trials, biomarker studies, and characterization of human pathogens are within the scope of the journal, as well as basic studies in model species such as Escherichia coli, Caenorhabditis elegans, Drosophila melanogaster, and Mus musculus revealing the function of molecules, cells, and tissues relevant for human health. However, articles on other species can be published if they contribute to our understanding of basic mechanisms of biology. A highly-cited international editorial board assures timely publication of manuscripts. Reviews on recent progress in biomedical sciences are commissioned by the editors.
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