1H, 13C and 15N backbone and sidechain assignment of the Burkholderia mallei acyl carrier protein

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2023-05-26 DOI:10.1007/s12104-023-10136-4

Srdan Matosin, Patrick D. Fischer, Maxim A. Droemer, Eric Baggs, Abu Sayeed Chowdhury, Isidoro Tavares, Scott B. Ficarro, Lisa Rose Warner, Haribabu Arthanari, Rajesh Nagarajan

引用次数: 0

Abstract

Acyl carrier proteins (ACPs) are universally conserved proteins amongst different species and are involved in fatty acid synthesis. Bacteria utilize ACPs as acyl carriers and donors for the synthesis of products such as endotoxins or acyl homoserine lactones (AHLs), which are used in quorum sensing mechanisms. In this study, wehave expressed isotopically labeled holo-ACP from Burkholderia mallei in Escherichia coli to assign 100% of non-proline backbone amide (HN) resonances, 95.5% of aliphatic carbon resonances and 98.6% of aliphatic hydrogen sidechain resonances.

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槌状伯克霍尔德菌酰基载体蛋白的1H、13C和15N主链和侧链分配

酰基载体蛋白（ACPs）是不同物种中普遍保守的蛋白质，参与脂肪酸的合成。细菌利用ACP作为酰基载体和供体合成产物，如内毒素或酰基高丝氨酸内酯（AHL），这些产物用于群体感应机制。在本研究中，我们在大肠杆菌中表达了来自马尔利伯克霍尔德菌的同位素标记的holo-ACP，以分配100%的非脯氨酸骨架酰胺（HN）共振、95.5%的脂族碳共振和98.6%的脂族氢侧链共振。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.