A label-free aptasensor for fluorescent detection of carbendazim based on a G-quadruplex-specific probe

IF 2.2 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2023-08-12 DOI:10.1007/s11696-023-03020-x

Lin Jiang, Lu Zhou, Jialin Sai, Dongguo Xue, Lijun Xu, Renjun Pei

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Abstract

Detection of carbendazim (CZ) fungicide residues in food has received increasing attention in recent years. However, most reported methods are sophisticated, high cost, and low throughput. In this study, we designed a label-free fluorescent aptasensor for detecting CZ by using a G-quadruplex-specific probe. The anti-CZ aptamer could be partially complementary to the guanine (G)-rich sequence PW17 and block the formation of G-quadruplex structures, while not in the presence of CZ. The formed G-quadruplex structure in the presence of CZ could non-covalently bind to the fluorescent probe N-methyl mesoporphyrin IX (NMM) and activate its fluorescence. A linear relationship between fluorescence and CZ was obtained in the concentration range of 0.05–0.4 μM with a detection limit of 24.28 nM. This study provides a new perspective for designing label-free aptasensors.

Graphical abstract

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基于G-四链体特异性探针的多菌灵荧光检测无标记适体传感器

近年来，食品中多菌灵杀菌剂残留的检测越来越受到重视。然而，大多数报道的方法都是复杂的、高成本的和低吞吐量的。在本研究中，我们设计了一种无标记荧光适体传感器，通过使用G-四链体特异性探针来检测CZ。抗CZ适体可以与富含鸟嘌呤（G）的序列PW17部分互补，阻断G-四链体结构的形成，而不存在CZ。在CZ存在下形成的G-四链结构可以与荧光探针N-甲基中卟啉IX（NMM）非共价结合并激活其荧光。在0.05–0.4μM的浓度范围内，荧光与CZ呈线性关系，检测限为24.28 nM。本研究为设计无标记适体传感器提供了新的视角。图形摘要

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来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.