Infectivity and genetic recombination of yellow mosaic viruses associated with blackgram (Vigna mungo L. Hepper) in South India

IF 0.9 4区农林科学 Q4 PLANT SCIENCES

Australasian Plant Pathology Pub Date : 2023-09-05 DOI:10.1007/s13313-023-00941-9

V. K. Satya, D. Alice, S. Kanakala, P. Jyothsna, V. G. Malathi, P. Jayamani

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Abstract

The pulse crops widely cultivated in South India for their protein rich grains are negatively affected by whitefly-transmitted begomoviruses (genus Begomovirus, family Geminiviridae) causing yellow mosaic disease (YMD). Here, YMD-inducing begomoviruses were characterized from cultivation areas in Tamil Nadu, South India, with complete sequences of seven DNA-A and eight DNA-B components determined. The pairwise nucleotide sequence comparisons for complete DNA-A showed that both mungbean yellow mosaic virus (MYMV) and mungbean yellow mosaic India virus (MYMIV) were present. The comparisons of nucleotide sequences of YMD begomoviral isolates revealed the presence of five groups of DNA-B components, which showed only 81–88% identity in the common region with their cognate DNA-A. The recombination analysis revealed significant events in the the non-coding and coding regions in DNA-B components, which were predicted amongst the MYMV isolates and between MYMV and MYMIV sequences. The partial tandem repeat constructs of MYMV and MYMIV DNA-A and DNA-B isolates (GenBank Accession Nos. KC911721, KC911724, AF142440 and AF126406) were infectious in blackgram (Vigna mungo), which showed typical YMD symptoms. Also the blackgram genotypes showed different levels of susceptibility and tolerance under experimental inoculation with MYMV and MYMIV. From the results, it is inferred that MYMV and MYMIV have occurred in mixed infection in blackgram.

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印度南部与黑斑病（Vigna mungo L.Hepper）相关的黄色花叶病毒的感染性和基因重组

南印度因其富含蛋白质的谷物而广泛种植的豆类作物受到白蝇传播的秋海棠病毒（秋海棠病毒属，双子座病毒科）的负面影响，该病毒会导致黄色花叶病（YMD）。在这里，对来自南印度泰米尔纳德邦种植区的YMD诱导秋海棠病毒进行了鉴定，确定了7个DNA-A和8个DNA-B组分的完整序列。完全DNA-A的成对核苷酸序列比较表明，绿豆黄花叶病毒（MYMV）和绿豆黄花叶病印度病毒（MYMIV）都存在。对YMD秋霉素分离株核苷酸序列的比较显示，存在五组DNA-B组分，它们在共同区域与同源DNA-A仅显示出81–88%的同一性。重组分析揭示了DNA-B组分中非编码区和编码区的重大事件，这些事件是在MYMV分离株之间以及MYMV和MYMIV序列之间预测的。MYMV和MYMIV DNA-A和DNA-B分离株（GenBank登录号：KC911721、KC911724、AF142440和AF126406）的部分串联重复构建体在黑色素瘤（Vigna mungo）中具有传染性，表现出典型的YMD症状。此外，在MYMV和MYMIV的实验接种下，黑图基因型表现出不同水平的易感性和耐受性。结果表明，MYMV和MYMIV均发生在黑色素瘤混合感染中。

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来源期刊

Australasian Plant Pathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions. Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.