Cellular evidence of communication strategies for small intestinal high endothelial cells: Ultrastructural insights of nano-scale exosomes and autophagy
Ping Hao , Wen Yin , Xi Chen , Shuangshuang Qin , Yue Yu , Yuan Yuan , Xiaoyu Quan , Bing Hu , Shouhai Chen , Yi Wu
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引用次数: 0
Abstract
Although several immune related cells of small intestine play an essential role in the intestinal homeostasis. However, information related to ultrastructural evidence of Nano-scale exosomes-multivesicular bodies and autophagic pathway in the high endothelial cells (HECs) of the small intestine in laying birds is still ambiguous. In present study, the HECs secreted the early endosome (ee), late endosome (le) and multivesicular bodies (MVBs) in the lamina propria of layer small intestine was confirmed by transmission electron microscopy. Besides that, in the cytoplasm of HECs showed many autophagosomes were directly associated with lysosomes and mitochondria. Further, the immunohistochemistry and immunofluorescence results showed that, the immunoreactivity and immuno-signaling of Nano-scale exosome related proteins, cluster of differentiation (CD63) and tumor susceptibility gene (TSG101), and autophagic related proteins, autophagic related gene (ATG7) and microtubule-associated protein light chain (LC3) were strong positive expression in the lamina propria of small intestine. These results prove that HECs play a well-known immunological role in the maintenance of intestinal homeostasis. In summary, these findings indicate that the small intestine’s HECs have developed an innovative way of communication.
期刊介绍:
Micron is an interdisciplinary forum for all work that involves new applications of microscopy or where advanced microscopy plays a central role. The journal will publish on the design, methods, application, practice or theory of microscopy and microanalysis, including reports on optical, electron-beam, X-ray microtomography, and scanning-probe systems. It also aims at the regular publication of review papers, short communications, as well as thematic issues on contemporary developments in microscopy and microanalysis. The journal embraces original research in which microscopy has contributed significantly to knowledge in biology, life science, nanoscience and nanotechnology, materials science and engineering.