Meconium Concentrations of Pesticides and Risk of Hypospadias: A Case-Control Study in Brittany, France.

IF 4.7 2区 医学 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH
Epidemiology Pub Date : 2024-03-01 Epub Date: 2023-11-02 DOI:10.1097/EDE.0000000000001688
Florence Rouget, Adèle Bihannic, Barbara Le Bot, Fabien Mercier, Erwann Gilles, Ronan Garlantezec, Luc Multigner, Sylvaine Cordier, Alexis Arnaud, Patrick Pladys, Cécile Chevrier
{"title":"Meconium Concentrations of Pesticides and Risk of Hypospadias: A Case-Control Study in Brittany, France.","authors":"Florence Rouget, Adèle Bihannic, Barbara Le Bot, Fabien Mercier, Erwann Gilles, Ronan Garlantezec, Luc Multigner, Sylvaine Cordier, Alexis Arnaud, Patrick Pladys, Cécile Chevrier","doi":"10.1097/EDE.0000000000001688","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hypospadias is a male genital tract defect for which an increase in prevalence has been documented over the last few decades. A role for environmental risk factors is suspected, including prenatal exposure to pesticides.</p><p><strong>Objectives: </strong>To study the risk of hypospadias in association with multiple pesticide measurements in meconium samples.</p><p><strong>Methods: </strong>The Brittany Registry of Congenital Anomalies (France) conducted a case-control study between 2012 and 2018. Cases were hypospadias, ascertained by a pediatrician and a pediatric surgeon, excluding genetic conditions, following European Surveillance of Congenital Anomalies guidelines (N = 69). Controls (N = 135) were two male infants without congenital anomaly born after each case in the same maternity unit. Mothers in the maternity units completed a self-administered questionnaire, we collected medical data from hospital records, and medical staff collected meconium samples. We performed chemical analysis of 38 pesticides (parent compound and/or metabolite) by UHPLC/MS/MS following strict quality assurance/quality control criteria and blind to case-control status. We carried out logistic regression accounting for frequency-matching variables and major risk factors.</p><p><strong>Results: </strong>Among the 38 pesticides measured, 16 (42%) were never detected in the meconium samples, 18 (47%) were in <5% of samples, and 4 (11%) in ≥5% of the samples. We observed an association between the detection of fenitrothion in meconium and the risk of hypospadias (OR = 2.6 [1.0-6.3] with n cases = 13, n controls = 21), but not the other pesticides.</p><p><strong>Conclusions: </strong>Our small study provides a robust assessment of fetal exposure. Fenitrothion's established antiandrogenic activities provide biologic plausibility for our observations. Further studies are needed to confirm this hypothesis.</p>","PeriodicalId":11779,"journal":{"name":"Epidemiology","volume":null,"pages":null},"PeriodicalIF":4.7000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epidemiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/EDE.0000000000001688","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/2 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Hypospadias is a male genital tract defect for which an increase in prevalence has been documented over the last few decades. A role for environmental risk factors is suspected, including prenatal exposure to pesticides.

Objectives: To study the risk of hypospadias in association with multiple pesticide measurements in meconium samples.

Methods: The Brittany Registry of Congenital Anomalies (France) conducted a case-control study between 2012 and 2018. Cases were hypospadias, ascertained by a pediatrician and a pediatric surgeon, excluding genetic conditions, following European Surveillance of Congenital Anomalies guidelines (N = 69). Controls (N = 135) were two male infants without congenital anomaly born after each case in the same maternity unit. Mothers in the maternity units completed a self-administered questionnaire, we collected medical data from hospital records, and medical staff collected meconium samples. We performed chemical analysis of 38 pesticides (parent compound and/or metabolite) by UHPLC/MS/MS following strict quality assurance/quality control criteria and blind to case-control status. We carried out logistic regression accounting for frequency-matching variables and major risk factors.

Results: Among the 38 pesticides measured, 16 (42%) were never detected in the meconium samples, 18 (47%) were in <5% of samples, and 4 (11%) in ≥5% of the samples. We observed an association between the detection of fenitrothion in meconium and the risk of hypospadias (OR = 2.6 [1.0-6.3] with n cases = 13, n controls = 21), but not the other pesticides.

Conclusions: Our small study provides a robust assessment of fetal exposure. Fenitrothion's established antiandrogenic activities provide biologic plausibility for our observations. Further studies are needed to confirm this hypothesis.

杀虫剂的胎粪浓度与尿道下裂风险:法国布列塔尼的一项病例对照研究。
背景:尿道下裂是一种男性生殖道缺陷,在过去几十年中,其患病率有所上升。怀疑环境风险因素的作用,包括产前接触杀虫剂。目的:研究胎粪样本中多种农药检测结果与尿道下裂的风险。方法:布列塔尼先天性畸形登记处(法国)在2012年至2018年间进行了一项病例对照研究。病例为尿道下裂,由儿科医生和儿科医生确定,不包括遗传疾病,遵循EUROCAT指南(N=69)。对照组(N=135)为两名无先天性畸形的男婴,每例病例后在同一产科病房出生。产科病房的母亲们完成了一份自我管理的问卷,我们从医院记录中收集了医疗数据,医务人员收集了胎粪样本。我们通过UHPLC/MS/MS对38种农药(母体化合物和/或代谢产物)进行了化学分析,遵循严格的质量保证/质量控制标准,并对病例对照状态视而不见。我们对频率匹配变量和主要风险因素进行了逻辑回归核算。结果:在测得的38种杀虫剂中,16种(42%)从未在胎粪样本中检测到,18种(47%)在胎粪中。结论:我们的小型研究提供了对胎儿暴露的有力评估。杀硝基硫磷已建立的抗雄激素活性为我们的观察提供了生物学上的合理性。需要进一步的研究来证实这一假设。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Epidemiology
Epidemiology 医学-公共卫生、环境卫生与职业卫生
CiteScore
6.70
自引率
3.70%
发文量
177
审稿时长
6-12 weeks
期刊介绍: Epidemiology publishes original research from all fields of epidemiology. The journal also welcomes review articles and meta-analyses, novel hypotheses, descriptions and applications of new methods, and discussions of research theory or public health policy. We give special consideration to papers from developing countries.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信