Interleukin-1β and TNF-α are elevated in the amygdala of adult rats prenatally exposed to ethanol.

Q3 Biochemistry, Genetics and Molecular Biology
V S Kokhan, P K Anokhin, T V Proskuryakova, V A Shokhonova, R A Ageldinov, I Yu Shamakina
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引用次数: 0

Abstract

Affective disorders, including anxiety and depression, developed in adult offspring of the mothers who consumed alcohol during pregnancy could be associated with an imbalance in neuroimmune factors in the amygdala (corpus amygdaloideum) resulted in impaired emotional stimulus processing. The aim of this study was to compare the content of cytokines TNF-α, IL-1α, IL-1β, IL-10, and IL-17 in the amygdala of adult female rats exposed to alcohol in utero and control rats. Cytokine levels were evaluated using a multiplex immunoassay system; mRNA expression was investigated using a real-time reverse transcription-polymerase chain reaction (RT-qPCR) assay. Prenatal alcohol exposure led to the increase in the content of TNF-α and IL-1β without significant changes in the mRNA expression level. Our data suggest that ethanol exposure to the fetus during pregnancy can result in long-term alterations in the content of the key neuroinflammatory factors in the amygdala, which in turn can be a risk factor for affective disorders in the adulthood.

出生前暴露于乙醇的成年大鼠杏仁核中白细胞介素-1β和TNF-α升高。
怀孕期间饮酒的母亲的成年子女出现的情感障碍,包括焦虑和抑郁,可能与杏仁核(杏仁核)神经免疫因子失衡有关,导致情绪刺激处理受损。本研究的目的是比较在子宫内暴露于酒精的成年雌性大鼠和对照大鼠杏仁核中细胞因子TNF-α、IL-1α、IL-2β、IL-10和IL-17的含量。使用多重免疫测定系统评估细胞因子水平;使用实时逆转录聚合酶链式反应(RT-qPCR)测定来研究mRNA表达。产前酒精暴露导致TNF-α和IL-1β含量增加,而mRNA表达水平没有显著变化。我们的数据表明,怀孕期间接触胎儿的乙醇会导致杏仁核中关键神经炎症因子含量的长期变化,这反过来又可能是成年期情感障碍的风险因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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