Contribution of the gasotransmitter nitric oxide to the structural and functional organization of erythrocytes under conditions of hypoxia/reoxygenation.

Q3 Biochemistry, Genetics and Molecular Biology

Biomeditsinskaya khimiya Pub Date : 2023-11-01 DOI:10.18097/PBMC20236905315

N V Akulich, V V Zinchuk

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Abstract

Hypoxia is accompanied by changes in metabolism and cell functioning. Erythrocyte hemoglobin can be involved in adaptation to hypoxia by acting as an oxygen sensor, providing a link between oxygen content and blood circulation. The mechanisms providing this function have not been completely established. The purpose of this study was to evaluate the effect of the gasotransmitter nitric oxide on the structural and functional organization of erythrocytes under conditions of hypoxia/reoxygenation. NO participated in adaptive reactions under hypoxia/reoxygenation conditions by changing hemoglobin conformation, followed by changes in hemoprotein spectral characteristics and hemoglobin affinity to oxygen together with increasing anisocytosis, volume and cell surface. The increase in intracellular NO concentrations under hypoxic conditions was provided by extracellular fluid nitrites. Molsidomine (a NO donor) induced a higher NO increase without involvement of the nitrite reductase mechanism, it caused an increase in the average erythrocyte volume, anisocytosis, and an increase in the cell surface.

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一氧化氮在缺氧/复氧条件下对红细胞结构和功能组织的贡献。

缺氧伴随着新陈代谢和细胞功能的变化。红细胞血红蛋白可以作为氧传感器参与对缺氧的适应，提供氧含量和血液循环之间的联系。提供这一功能的机制尚未完全建立。本研究的目的是评估在缺氧/复氧条件下，气体发射器一氧化氮对红细胞结构和功能组织的影响。NO通过改变血红蛋白构象参与缺氧/复氧条件下的适应性反应，随后改变血蛋白光谱特征和血红蛋白对氧的亲和力，同时增加细胞异径、体积和细胞表面。缺氧条件下细胞内NO浓度的增加是由细胞外液体亚硝酸盐提供的。Molsidomine（一种NO供体）在不参与亚硝酸盐还原酶机制的情况下诱导了更高的NO增加，它引起了平均红细胞体积的增加、异细胞增多和细胞表面的增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).