Nano/microfluidic device for high-throughput passive trapping of nanoparticles.

IF 2.6 4区工程技术 Q2 BIOCHEMICAL RESEARCH METHODS

Biomicrofluidics Pub Date : 2023-11-01 eCollection Date: 2023-12-01 DOI:10.1063/5.0176323

Tanner Wells, Holger Schmidt, Aaron Hawkins

引用次数: 0

Abstract

We present a design and a fabrication method for devices designed for rapid collection of nanoparticles in a fluid. The design uses nanofluidic channels as a passive size-based barrier trap to isolate particles near a central point in the channel, which is also covered by a thin membrane. Particles that enter the collection region are trapped with 100% efficiency within a 6-12 $μ$ m radius from a central point. Flow rates for particle-free fluid range from 1.88 to 3.69 nl/s for the pressure and geometries tested. Particle trapping tests show that high trapped particle counts significantly impact flow rates. For suspensions as dilute as 30-300 aM (20-200 particles/ $μ$ l), 8-80 particles are captured within 500 s.

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用于高通量被动捕获纳米颗粒的纳米/微流体装置。

我们提出了一种用于在流体中快速收集纳米颗粒的装置的设计和制造方法。该设计使用纳米流体通道作为基于尺寸的被动阻挡阱，以隔离通道中中心点附近的颗粒，该中心点也被薄膜覆盖。进入收集区域的颗粒在6-12 μm半径。无颗粒流体的流速范围为1.88至3.69 nl/s，用于测试的压力和几何形状。颗粒捕获测试表明，高捕获颗粒数显著影响流速。对于稀释至30-300的悬浮液 aM（20-200个粒子/μl），在500范围内捕获8-80个粒子 s

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomicrofluidics 生物-纳米科技

CiteScore

5.80

自引率

3.10%

发文量

审稿时长

1.3 months

期刊介绍： Biomicrofluidics (BMF) is an online-only journal published by AIP Publishing to rapidly disseminate research in fundamental physicochemical mechanisms associated with microfluidic and nanofluidic phenomena. BMF also publishes research in unique microfluidic and nanofluidic techniques for diagnostic, medical, biological, pharmaceutical, environmental, and chemical applications. BMF offers quick publication, multimedia capability, and worldwide circulation among academic, national, and industrial laboratories. With a primary focus on high-quality original research articles, BMF also organizes special sections that help explain and define specific challenges unique to the interdisciplinary field of biomicrofluidics. Microfluidic and nanofluidic actuation (electrokinetics, acoustofluidics, optofluidics, capillary) Liquid Biopsy (microRNA profiling, circulating tumor cell isolation, exosome isolation, circulating tumor DNA quantification) Cell sorting, manipulation, and transfection (di/electrophoresis, magnetic beads, optical traps, electroporation) Molecular Separation and Concentration (isotachophoresis, concentration polarization, di/electrophoresis, magnetic beads, nanoparticles) Cell culture and analysis(single cell assays, stimuli response, stem cell transfection) Genomic and proteomic analysis (rapid gene sequencing, DNA/protein/carbohydrate arrays) Biosensors (immuno-assay, nucleic acid fluorescent assay, colorimetric assay, enzyme amplification, plasmonic and Raman nano-reporter, molecular beacon, FRET, aptamer, nanopore, optical fibers) Biophysical transport and characterization (DNA, single protein, ion channel and membrane dynamics, cell motility and communication mechanisms, electrophysiology, patch clamping). Etc...