Development of in situ crosslinked hyaluronan as an adjunct to vitrectomy surgery

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2023-11-06 DOI:10.1007/s10856-023-06757-9

Kiyoshi Suzuki, Ippei Watanabe, Takashi Tachibana, Kenichiro Mori, Keijiro Ishikawa, Tatsuro Ishibashi, Eiichi Uchio, Koh-Hei Sonoda, Toshio Hisatomi

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Abstract

Ophthalmologists have used hyaluronan (HA) products as adjuncts to ocular surgery since the 1970s. However, HA products are not always functional in surgeries of the posterior eye segment due to their lack of biomechanical strength. In this study, we developed an in situ crosslinked HA (XL-HA) and evaluated its potential as an adjunct to vitrectomy surgery in an in vitro model with a triamcinolone acetonide (TA) layer used as a pseudo residual vitreous cortex (RVC). Within a few minutes at concentrations over 0.9%, XL-HA, generated by the click chemistry of HA-dibenzocyclooctyne and HA-azidoethylamine, formed a hydrogel with the appropriate hardness for tweezers peeling. XL-HA (concentration, 0.76–1.73%) without dispersion successfully entered the TA layer and removed more than 45% of the total TA. Dynamic viscoelasticity helps to explain the rheological behavior of hydrogels, and the assessment results for XL-HA indicated that suitable concentrations were between 0.97% and 1.30%. For example, 1.30% XL-HA hydrogel reached sufficient hardness at 3 min for tweezers peeling, and the TA removal ability exceeded 70%. These results demonstrated that XL-HA was a potential adjunct to successful vitrectomy.

Graphical Abstract

Abstract Image

查看原文本刊更多论文

原位交联透明质酸作为玻璃体切割手术辅助剂的发展。

自20世纪70年代以来，眼科医生一直使用透明质酸（HA）产品作为眼科手术的辅助药物。然而，由于缺乏生物力学强度，HA产品在后眼部手术中并不总是有效的。在本研究中，我们开发了一种原位交联HA（XL-HA），并在体外模型中评估了其作为玻璃体切除手术辅助物的潜力，该模型将曲安奈德（TA）层用作假残留玻璃体皮层（RVC）。在浓度超过0.9%的几分钟内，由HA二苯并环辛烯和HA叠氮乙胺的点击化学产生的XL-HA形成了具有适当硬度的水凝胶，用于镊子剥离。没有分散的XL-HA（浓度，0.76-1.73%）成功地进入TA层，并去除了超过45%的总TA。动态粘弹性有助于解释水凝胶的流变行为，XL-HA的评估结果表明，合适的浓度在0.97%和1.30%之间。例如，1.30%的XL-HA水凝胶在3 分钟，并且TA去除能力超过70%。这些结果表明XL-HA是成功玻璃体切除术的潜在辅助手段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.