CircRNA circ_0013339 Regulates the Progression of Colorectal Cancer Through miR-136-5p/SOX9 Axis

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2023-11-05 DOI:10.1007/s10528-023-10540-4

Juan Jin, Min Du, Ding Ding, Ran Xuan

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Abstract

Background

Colorectal cancer (CRC) is a common gastrointestinal malignancy. Dysregulation of circular RNAs (circRNAs) is associated with the progression of CRC. However, the role of circ_0013339 (hsa_circ_0013339) in CRC is still not clear.

Methods

The levels of circ_0013339, miR-136-5p, and SRY-box transcription factor 9 (SOX9) in CRC were gauged by quantitative real-time polymerase chain reaction (qRT-PCR). Colony formation and 5-Ethynyl-2’-deoxyuridine (EdU) assays were used to detect cell proliferation. Cell counting kit-8 (CCK8) assay was used to measure cell viability. Western blot assay was performed to examine protein expression. The relationship between miR-136-5p and circ_0013339 or SOX9 was tested by dual-luciferase reporter assay. The effect of sh-circ_0013339 on tumor growth in vivo was examined by xenograft experiments.

Results

Circ_0013339 expression was elevated in CRC tissues and cells, and circ_0013339 knockdown diminished the growth of CRC cells. MiR-136-5p was regulated by circ_0013339. MiR-136-5p deficiency ameliorated the effects of circ_0013339 silencing on CRC cell malignant behaviors. Circ_0013339 modulated SOX9 expression through miR-136-5p. SOX9 addition reversed the effects of miR-136-5p overexpression on CRC cell behaviors. Moreover, silencing of circ_0013339 suppressed the growth of xenograft tumors in vivo.

Conclusion

Circ_0013339 regulates the progression of CRC through miR-136-5p-dependent regulation of SOX9, uncovering a novel regulatory mechanism of circ_0013339 in CRC.

Abstract Image

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CircRNA circ_0013339通过miR-136-5p/SOX9轴调节结直肠癌癌症的进展。

背景：癌症是一种常见的胃肠道恶性肿瘤。环状RNA（circRNAs）的失调与CRC的进展有关。然而，circ_0013339（hsa_cir_0013339）在CRC中的作用仍然不清楚。方法：采用实时定量聚合酶链反应（qRT-PCR）检测CRC中circ_0013339、miR-136-5p和SRY-box转录因子9（SOX9）的水平。集落形成和5-乙炔基-2'-脱氧尿苷（EdU）测定用于检测细胞增殖。细胞计数试剂盒-8（CCK8）测定法用于测定细胞活力。进行蛋白质印迹分析以检测蛋白质表达。miR-136-5p与circ_0013339或SOX9之间的关系通过双荧光素酶报告基因测定进行测试。通过异种移植物实验检测了sh-circ_003339对体内肿瘤生长的影响。结果：Circ_0013339在CRC组织和细胞中的表达升高，而Circ_0013339的敲低降低了CRC细胞的生长。MiR-136-5p受circ_0013339的调节。MiR-136-5p缺乏改善了circ_0013339沉默对CRC细胞恶性行为的影响。Circ_0013339通过miR-136-5p调节SOX9的表达。SOX9的添加逆转了miR-136-5p过表达对CRC细胞行为的影响。此外，circ_0013339的沉默抑制了体内异种移植物肿瘤的生长。结论：Circ_0013339通过miR-136-5p依赖性调节SOX9来调节CRC的进展，揭示了Circ_001339在CRC中的新调控机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.