Assessment of Matrix Metalloprotease - 7 (MMP7) Immunohistochemistry in Biliary Atresia and Other Pediatric Cholestatic Liver Diseases.

IF 0.7 4区医学 Q4 PATHOLOGY

Fetal and Pediatric Pathology Pub Date : 2024-09-01 Epub Date: 2023-10-31 DOI:10.1080/15513815.2023.2276780

Sandhya Biswal, Dipanwita Biswas, Santosh Kumar Mahalik, Suvendu Purkait, Suvradeep Mitra

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引用次数: 0

Abstract

Background and aims: Biliary atresia (BA) is a progressive fibro-obliterative cholangiopathy. The histopathological diagnosis is often challenging and an immunohistochemical marker is often sought as an adjunct. We evaluated MMP7 immunohistochemistry in BA and other non-BA pediatric cholestatic liver diseases. Materials and methods: MMP7 immunohistochemistry was applied in 5 age-matched normal control, 23 cases of BA and 43 cases of non-BA pediatric cholestasis including 16 cases of choledochal cyst (CC), and a multiplication score was obtained by multiplying the intensity and percentage positivity in the cholangiocytes. Results: BA showed a high mean MMP7 multiplication score which was significantly different from the normal control and other non-BA pediatric cholestatic diseases including CC (p value < 0.001). The sensitivity, specificity, positive, and negative predictive values of MMP7 immunohistochemistry were 91.3%, 93.02%, 87.5%, and 95.2% respectively. Conclusion: MMP7 immunohistochemistry may be an adjunct to histomorphology in BA.

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基质金属蛋白酶-7（MMP7）免疫组织化学在胆道闭锁和其他儿童胆汁淤积性肝病中的评估。

背景与目的：胆道闭锁（BA）是一种进行性纤维闭塞性胆管疾病。组织病理学诊断通常具有挑战性，并且经常寻求免疫组织化学标记物作为辅助手段。我们评估了MMP7免疫组织化学在BA和其他非BA儿童胆汁淤积性肝病中的作用。材料和方法：应用MMP7免疫组化方法对5例年龄匹配的正常对照、23例BA和43例非BA儿童胆汁淤积症（包括16例胆总管囊肿）进行免疫组化，并通过乘以胆管细胞中的阳性强度和阳性百分比来获得倍增得分。结果：BA的MMP7平均增殖评分较高，与正常对照组和包括CC在内的其他非BA儿童胆汁淤积性疾病有显著差异（p值<0.001）。MMP7免疫组化的敏感性、特异性、阳性和阴性预测值分别为91.3%、93.02%、87.5%和95.2%。结论：MMP7免疫组化可能是BA组织形态学的辅助手段。

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来源期刊

Fetal and Pediatric Pathology 医学-病理学

CiteScore

3.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Fetal and Pediatric Pathology is an established bimonthly international journal that publishes data on diseases of the developing embryo, newborns, children, and adolescents. The journal publishes original and review articles and reportable case reports. The expanded scope of the journal encompasses molecular basis of genetic disorders; molecular basis of diseases that lead to implantation failures; molecular basis of abnormal placentation; placentology and molecular basis of habitual abortion; intrauterine development and molecular basis of embryonic death; pathogenisis and etiologic factors involved in sudden infant death syndrome; the underlying molecular basis, and pathogenesis of diseases that lead to morbidity and mortality in newborns; prenatal, perinatal, and pediatric diseases and molecular basis of diseases of childhood including solid tumors and tumors of the hematopoietic system; and experimental and molecular pathology.