Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes.

IF 3.6 Q1 TROPICAL MEDICINE
Daisuke Kobayashi, Izumi Kai, Astri Nur Faizah, Meng Ling Moi, Shigeru Tajima, Tomohiko Takasaki, Toshinori Sasaki, Haruhiko Isawa
{"title":"Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes.","authors":"Daisuke Kobayashi,&nbsp;Izumi Kai,&nbsp;Astri Nur Faizah,&nbsp;Meng Ling Moi,&nbsp;Shigeru Tajima,&nbsp;Tomohiko Takasaki,&nbsp;Toshinori Sasaki,&nbsp;Haruhiko Isawa","doi":"10.1186/s41182-023-00553-5","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV in the country, but knowledge of its viral susceptibility is limited. Therefore, we aimed to clarify the status of DENV susceptibility by comparing the infection and dissemination dynamics of Japanese Ae. albopictus to all known DENV serotypes with those of Ae. aegypti.</p><p><strong>Methods: </strong>After propagation of each DENV serotype in Vero cells, the culture supernatants were mixed with defibrinated rabbit blood and adenosine triphosphate, and the mixture was artificially blood-sucked by two colonies of Ae. albopictus from Japan and one colony of Ae. aegypti from a dengue-endemic country (Vietnam). After 14 days of sucking, the mosquito body was divided into two parts (thorax/abdomen and head/wings/legs) and total RNA was extracted from each sample. DENV RNA was detected in these extracted RNA samples using a quantitative RT-PCR method specific for each DENV serotype, and infection and dissemination rates were analyzed.</p><p><strong>Results: </strong>The Japanese Ae. albopictus colonies were susceptible to all DENV serotypes. Its infection and dissemination rates were significantly lower than those of Ae. aegypti. However, the number of DENV RNA copies in Ae. albopictus was almost not significantly different from that in Ae. aegypti. Furthermore, Japanese Ae. albopictus differed widely in their susceptibility to each DENV serotype.</p><p><strong>Conclusions: </strong>In Japanese Ae. albopictus, once DENV overcame the midgut infection barrier, the efficiency of subsequent propagation and dissemination of the virus in the mosquito body was comparable to that of Ae. aegypti. Based on the results of this study and previous dengue outbreak trends, Ae. albopictus is predicted to be highly compatible with DENV-1, suggesting that this serotype poses a high risk for future epidemics in Japan.</p>","PeriodicalId":23311,"journal":{"name":"Tropical Medicine and Health","volume":"51 1","pages":"61"},"PeriodicalIF":3.6000,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10621184/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Medicine and Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s41182-023-00553-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"TROPICAL MEDICINE","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV in the country, but knowledge of its viral susceptibility is limited. Therefore, we aimed to clarify the status of DENV susceptibility by comparing the infection and dissemination dynamics of Japanese Ae. albopictus to all known DENV serotypes with those of Ae. aegypti.

Methods: After propagation of each DENV serotype in Vero cells, the culture supernatants were mixed with defibrinated rabbit blood and adenosine triphosphate, and the mixture was artificially blood-sucked by two colonies of Ae. albopictus from Japan and one colony of Ae. aegypti from a dengue-endemic country (Vietnam). After 14 days of sucking, the mosquito body was divided into two parts (thorax/abdomen and head/wings/legs) and total RNA was extracted from each sample. DENV RNA was detected in these extracted RNA samples using a quantitative RT-PCR method specific for each DENV serotype, and infection and dissemination rates were analyzed.

Results: The Japanese Ae. albopictus colonies were susceptible to all DENV serotypes. Its infection and dissemination rates were significantly lower than those of Ae. aegypti. However, the number of DENV RNA copies in Ae. albopictus was almost not significantly different from that in Ae. aegypti. Furthermore, Japanese Ae. albopictus differed widely in their susceptibility to each DENV serotype.

Conclusions: In Japanese Ae. albopictus, once DENV overcame the midgut infection barrier, the efficiency of subsequent propagation and dissemination of the virus in the mosquito body was comparable to that of Ae. aegypti. Based on the results of this study and previous dengue outbreak trends, Ae. albopictus is predicted to be highly compatible with DENV-1, suggesting that this serotype poses a high risk for future epidemics in Japan.

Abstract Image

Abstract Image

埃及伊蚊和日本白纹伊蚊对所有登革热病毒血清型易感性的比较分析。
背景:登革热是由登革病毒(DENV)引起的,是全球最常见的由伊蚊(主要是埃及伊蚊和白纹伊蚊)传播的病毒感染。埃及伊蚊目前尚未在日本建立,白纹伊蚊是该国DENV的主要媒介蚊子,但对其病毒易感性的了解有限。因此,我们的目的是通过比较日本白纹伊蚊对所有已知DENV血清型和埃及伊蚊的感染和传播动态来阐明DENV的易感性状况。方法:每种DENV血清型在Vero细胞中繁殖后,将培养上清液与去纤维兔血和三磷酸腺苷混合,并由两个来自日本的白纹伊蚊和一个来自登革热流行国(越南)的埃及伊蚊人工吸血。吮吸14天后,将蚊子的身体分为两部分(胸部/腹部和头部/翅膀/腿),并从每个样本中提取总RNA。使用对每个DENV血清型特异的定量RT-PCR方法在这些提取的RNA样品中检测DENV RNA,并分析感染率和传播率。结果:日本白纹伊蚊群体对所有DENV血清型均敏感。其感染率和传播率明显低于埃及伊蚊。然而,白纹伊蚊的DENV RNA拷贝数与埃及伊蚊几乎没有显著差异。此外,日本白纹伊蚊对每种登革病毒血清型的易感性差异很大。结论:在日本白纹伊蚊中,DENV一旦克服中肠感染屏障,病毒在蚊子体内的繁殖和传播效率与埃及伊蚊相当。根据这项研究的结果和以前登革热爆发的趋势,白纹伊蚊被预测与DENV-1高度兼容,这表明该血清型在日本未来流行的风险很高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Tropical Medicine and Health
Tropical Medicine and Health TROPICAL MEDICINE-
CiteScore
7.00
自引率
2.20%
发文量
90
审稿时长
11 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信