The molybdenum cofactor biosynthesis gene, OsCNX1, is essential for seedling development and seed germination in rice.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2023-10-26 eCollection Date: 2023-11-01 DOI:10.1007/s11032-023-01424-x

Xiaoguang Lu, Di Zhang, Yi Zhang, Xing Liu, Sheng Wang, Xin Liu

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Abstract

Pre-harvest sprouting (PHS) frequently occurs in rice due to the long spells of rainy weather, and causes severe yield loss and grain quality decrease. Here, we identified one PHS-related gene OsCNX1 cloned from rice PHS mutant, which encoded a molybdenum cofactor (MoCo) biosynthesis enzyme. Genetic complementation indicated OsCNX1 could rescue the PHS and seedling lethal phenotype of the mutant. Expression pattern showed that OsCNX1 was expressed in rice tissue including seedling shoot, culm, blade, and sheath of flag leaf, young panicle, and the seeds at different development stages. Overexpression of OsCNX1 significantly decreased the plant height, and the seed germination of the dormant seeds harvested from fresh panicles, comparing to the wild type (WT). In addition, 1492 differentially expressed genes (DEGs) were identified between OsCNX1-overexpressed line and WT by RNA-sequencing, which were mainly classified in plant-pathogen interaction, plant hormone signal transduction, and starch/sucrose metabolism. These results showed that OsCNX1 was not only necessary for rice seed germination, but also participated in plant development, indicating that OsCNX1 may be useful in rice breeding of PHS resistance and plant height.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-023-01424-x.

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钼辅因子生物合成基因OsCNX1对水稻幼苗发育和种子发芽至关重要。

由于长时间的阴雨天气，水稻收获前发芽（PHS）频繁发生，造成严重的产量损失和稻米品质下降。在这里，我们鉴定了从水稻PHS突变体中克隆的一个PHS相关基因OsCNX1，该基因编码钼辅因子（MoCo）生物合成酶。遗传互补表明OsCNX1可以挽救突变体的PHS和幼苗致死表型。表达模式表明，OsCNX1在水稻幼苗芽、茎、叶片、旗叶鞘、幼穗和不同发育阶段的种子组织中均有表达。与野生型（WT）相比，OsCNX1的过表达显著降低了从新鲜圆锥花序收获的休眠种子的株高和种子发芽率。此外，通过RNA测序，在OsCNX1过表达系和WT之间鉴定出1492个差异表达基因，主要分为植物-病原体相互作用、植物激素信号转导和淀粉/蔗糖代谢。这些结果表明，OsCNX1不仅是水稻种子发芽所必需的，而且参与了植株的发育，表明OsCNX1可能有助于水稻PHS抗性和株高的育种。补充信息：在线版本包含补充材料，可访问10.1007/s11032-023-01424-x。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.