Imaging the binding of MECP2 to DNA.

IF 7.5 1区 生物学 Q1 CELL BIOLOGY
Genes & development Pub Date : 2023-10-01 Epub Date: 2023-11-01 DOI:10.1101/gad.351285.123
Anne E West
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引用次数: 0

Abstract

Mutations in the methyl-DNA binding domain of MECP2 cause Rett syndrome; however, distinct mutations are associated with different severity of the disease. Live-cell imaging and single-molecule tracking are sensitive methods to quantify the DNA binding affinity and diffusion dynamics of nuclear proteins. In this issue of Genes & Development, Zhou and colleagues (pp. 883-900) used these imaging methods to quantitatively describe the partial loss of DNA binding resulting from a novel pathological MECP2 mutation with intermediate disease severity. These data demonstrate how single-molecule tracking can advance understanding of the molecular mechanisms connecting MECP2 mutations with Rett syndrome pathophysiology.

对MECP2与DNA的结合进行成像。
MECP2甲基DNA结合结构域的突变导致Rett综合征;然而,不同的突变与疾病的不同严重程度有关。活细胞成像和单分子追踪是量化核蛋白DNA结合亲和力和扩散动力学的敏感方法。在本期《基因与发育》杂志上,周及其同事(第XXX-XXX页)使用这些成像方法定量描述了一种新的病理性MECP2突变导致的DNA结合的部分丧失,该突变具有中等疾病严重程度。这些数据证明了单分子追踪如何促进对MECP2突变与Rett综合征病理生理学联系的分子机制的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Genes & development
Genes & development 生物-发育生物学
CiteScore
17.50
自引率
1.90%
发文量
71
审稿时长
3-6 weeks
期刊介绍: Genes & Development is a research journal published in association with The Genetics Society. It publishes high-quality research papers in the areas of molecular biology, molecular genetics, and related fields. The journal features various research formats including Research papers, short Research Communications, and Resource/Methodology papers. Genes & Development has gained recognition and is considered as one of the Top Five Research Journals in the field of Molecular Biology and Genetics. It has an impressive Impact Factor of 12.89. The journal is ranked #2 among Developmental Biology research journals, #5 in Genetics and Heredity, and is among the Top 20 in Cell Biology (according to ISI Journal Citation Reports®, 2021).
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