Phylogenetic position of the pelobiont Mastigamoeba aspera and revision of the family Mastigamoebidae (Archamoebae, Pelobiontida)

IF 1.9 2区生物学 Q4 MICROBIOLOGY

European journal of protistology Pub Date : 2023-10-01 DOI:10.1016/j.ejop.2023.126029

Ludmila V. Chistyakova , Alexei Yu. Kostygov , Andrew V. Goodkov , Anton V. Radaev , Alexander O. Frolov

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Abstract

In this study, we presented the results of our molecular phylogenetic analysis of Archamoebae using a newly obtained sequence of the 18S rRNA gene of Mastigamoeba aspera, the type species of the genus. In addition, we characterized the general organization of the tubulin cytoskeleton of M. aspera by immunofluorescent microscopy and TEM. Our findings allowed us to revise the family Mastigamoebidae and establish within it two subfamilies: Mastigamoebinae for the genus Mastigamoeba and Seraviniinae subfam. nov. for the genera Seravinia gen. nov., Paramastigamoeba gen. nov., Iodamoeba and Endolimax. The type genera Mastigamoeba and Seravinia are clearly distinguished by the structure of the basal apparatus of flagella. In addition, the tubulin cytoskeleton of several Mastigamoeba spp. contains a rim of microtubules around the nuclei.

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Mastigamoeba aspera pelobiont的系统发育位置和Mastigamoebidae科（Archamoebae，Pelobiotida）的修订。

在这项研究中，我们使用最新获得的Mastigamoeba aspera（该属的模式种）的18S rRNA基因序列，对Archamoebae进行了分子系统发育分析。此外，我们还通过免疫荧光显微镜和透射电镜对M.aspera微管蛋白细胞骨架的一般组织进行了表征。我们的发现使我们能够修改Mastigamoebidae家族，并在其中建立两个亚科：Mastigamoeba属的Mastigamoebenae和Seraviniinae亚科。Seravinia属，Paramastigamoeba属，碘虫属和Endolimax属。Mastigamoeba和Seravinia模式属通过鞭毛的基器结构可以清楚地区分。此外，几种Mastigamoeba属的微管蛋白细胞骨架在细胞核周围含有一圈微管。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European journal of protistology 生物-微生物学

CiteScore

4.60

自引率

20.70%

发文量

审稿时长

14.6 weeks

期刊介绍： Articles deal with protists, unicellular organisms encountered free-living in various habitats or as parasites or used in basic research or applications. The European Journal of Protistology covers topics such as the structure and systematics of protists, their development, ecology, molecular biology and physiology. Beside publishing original articles the journal offers a forum for announcing scientific meetings. Reviews of recently published books are included as well. With its diversity of topics, the European Journal of Protistology is an essential source of information for every active protistologist and for biologists of various fields.