A comparison of osteogenic effect of newly manufactured calcium silicate-based sealers in vitro.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental materials journal Pub Date : 2023-11-29 Epub Date: 2023-10-31 DOI:10.4012/dmj.2023-048

Kkot-Byeol Bae, Yoorina Choi, Bin-Na Lee, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang

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Abstract

This study aimed to assess the effect of different calcium silicate-based root canal sealers (CSRS) on osteogenic effect in human periodontal ligament cells (hPDLCs). hPDLCs were cultured in a medium containing extract of 5 types of CSRS. The specimens were assessed by the cell cytotoxicity test, alkaline phosphatase staining, alizarin red S staining, quantitative real-time PCR, Western blot analysis, and enzyme-linked immunosorbent assay. The diluted concentrations of extracted solutions had no significant effect on the viability of hPDLCs. There was a statistically significant difference in the mRNA expression level of bone sialoprotein (BSP), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2) among some groups. The protein expressions of BSP, OCN, and RUNX2 were significantly higher in some groups compared to the control group. The CSRS did not interfere with the osteogenic differentiation of hPDLCs, compared to the control group. CSRS are shown to have biocompatibility and osteogenic differentiation effect on hPDLCs.

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新型硅酸钙基封闭剂体外成骨效果的比较。

本研究旨在评估不同硅酸钙基根管封闭剂（CSRS）对人牙周膜细胞（hPDLC）成骨作用的影响。hPDLC在含有5种类型的CSRS的提取物的培养基中培养。通过细胞毒性试验、碱性磷酸酶染色、茜素红S染色、实时定量PCR、蛋白质印迹分析和酶联免疫吸附试验对标本进行评估。提取溶液的稀释浓度对hPDLC的活力没有显著影响。骨唾液蛋白（BSP）、骨钙素（OCN）和runt相关转录因子2（RUNX2）的mRNA表达水平在某些组之间存在统计学显著差异。与对照组相比，某些组的BSP、OCN和RUNX2的蛋白表达显著更高。与对照组相比，CSRS不干扰hPDLC的成骨分化。CSRS对hPDLC具有生物相容性和成骨分化作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental materials journal 医学-材料科学：生物材料

CiteScore

4.60

自引率

4.00%

发文量

102

审稿时长

3 months

期刊介绍： Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.