Incorporating zinc ion into titanium surface promotes osteogenesis and osteointegration in implantation early phase

IF 4.2 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Materials Science: Materials in Medicine Pub Date : 2023-11-02 DOI:10.1007/s10856-023-06751-1

Xutengyue Tian, Peng Zhang, Juan Xu

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引用次数: 0

Abstract

The objective of this study is to further investigate the feasibility of Zinc–Titanium implant as a potential implantable material in oral application in aspects of osteoblast biocompatibility, osteogenesis and osseointegration ability. First, we used plasma immersion ion implantation and deposition (PIIID) technology to introduce Zinc ion into pure Titanium surface, then we used X-ray photoelectron spectroscopy to analyze the chemical composition of modified surface layer; next, we used in vitro studies including immunological fluorescence assay and western blotting to determine responses between MG-63 osteoblast-like cell and implant. In vivo studies adopted pig model to check the feasibility of Zn–Ti implant. Results showed that in vitro and in vivo were consistent, showing that Zn ion was successfully introduced into Ti surface by PIIID technique. The chemical and physical change on modified plant resulted in the more active expressions of mRNA and protein of Type I collagen in MG-63 cells compared with non-treated implant, and the better integration ability of bones with modified implant. We confirmed the Zn–Ti implant owns the ability in promoting osteogenesis and osteointegration in early phase of implantation and is a qualified candidate in dentistry. The overview of our study can be depicted as follows.

Graphical Abstract

Abstract Image

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将锌离子掺入钛表面可促进植入早期的成骨和骨整合。

本研究的目的是从成骨细胞的生物相容性、成骨和骨整合能力等方面进一步探讨锌钛种植体作为一种潜在的口腔植入材料的可行性。首先，我们使用等离子体浸没离子注入和沉积（PIIID）技术将锌离子引入纯钛表面，然后使用X射线光电子能谱分析改性表面层的化学成分；接下来，我们使用包括免疫荧光分析和蛋白质印迹在内的体外研究来确定MG-63成骨细胞样细胞和植入物之间的反应。体内研究采用猪模型来检查锌钛植入物的可行性。结果表明，体外和体内实验结果一致，表明利用PIIID技术成功地将Zn离子引入Ti表面。与未处理的植入物相比，修饰植物的化学和物理变化导致MG-63细胞中I型胶原的mRNA和蛋白表达更活跃，并且修饰植入物具有更好的骨整合能力。我们证实锌钛植入物在植入早期具有促进成骨和骨整合的能力，是牙科的合格候选者。我们的研究概述如下。

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来源期刊

Journal of Materials Science: Materials in Medicine 工程技术-材料科学：生物材料

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

3.5 months

期刊介绍： The Journal of Materials Science: Materials in Medicine publishes refereed papers providing significant progress in the application of biomaterials and tissue engineering constructs as medical or dental implants, prostheses and devices. Coverage spans a wide range of topics from basic science to clinical applications, around the theme of materials in medicine and dentistry. The central element is the development of synthetic and natural materials used in orthopaedic, maxillofacial, cardiovascular, neurological, ophthalmic and dental applications. Special biomedical topics include biomaterial synthesis and characterisation, biocompatibility studies, nanomedicine, tissue engineering constructs and cell substrates, regenerative medicine, computer modelling and other advanced experimental methodologies.