PbrChiA: a key chitinase of pear in response to Botryosphaeria dothidea infection by interacting with PbrLYK1b2 and down-regulating ROS accumulation.

IF 7.6 Q1 GENETICS & HEREDITY
园艺研究(英文) Pub Date : 2023-09-19 eCollection Date: 2023-10-01 DOI:10.1093/hr/uhad188
Qiming Chen, Huizhen Dong, Qionghou Li, Xun Sun, Xin Qiao, Hao Yin, Zhihua Xie, Kaijie Qi, Xiaosan Huang, Shaoling Zhang
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Abstract

Pear ring rot, caused by the pathogenic fungi Botryosphaeria dothidea, seriously affects pear production. While the infection-induced reactive oxygen species (ROS) burst of infected plants limits the proliferation of B. dothidea during the early infection stage, high ROS levels can also contribute to their growth during the later necrotrophic infection stage. Therefore, it is important to understand how plants balance ROS levels and resistance to pathogenic B. dothidea during the later stage. In this study, we identified PbrChiA, a glycosyl hydrolases 18 (GH18) chitinase-encoding gene with high infection-induced expression, through a comparative transcriptome analysis. Artificial substitution, stable overexpression, and virus induced gene silencing (VIGS) experiments demonstrated that PbrChiA can positively regulate pear resistance as a secreted chitinase to break down B. dothidea mycelium in vitro and that overexpression of PbrChiA suppressed infection-induced ROS accumulation. Further analysis revealed that PbrChiA can bind to the ectodomain of PbrLYK1b2, and this interaction suppressed PbrLYK1b2-mediated chitin-induced ROS accumulation. Collectively, we propose that the combination of higher antifungal activity from abundant PbrChiA and lower ROS levels during later necrotrophic infection stage confer resistance of pear against B. dothidea.

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PbrChiA:一种关键的梨几丁质酶,通过与PbrLYK1b2相互作用并下调ROS的积累来应对斑点球孢菌的感染。
梨环腐病是由病原真菌梨孢菌引起的,严重影响梨的生产。虽然受感染植物的感染诱导的活性氧(ROS)爆发在感染早期限制了B.dothidea的增殖,但高ROS水平也有助于它们在后期坏死营养感染阶段的生长。因此,了解植物在后期如何平衡ROS水平和对致病性斑点线虫的抗性是很重要的。在本研究中,我们通过比较转录组分析鉴定了PbrChiA,一种编码糖基水解酶18(GH18)几丁质酶的基因,具有高感染诱导表达。人工替代、稳定过表达和病毒诱导的基因沉默(VIGS)实验表明,PbrChiA可以作为一种分泌的几丁质酶在体外分解B.dothidea菌丝体,积极调节梨的抗性,并且PbrChiA的过表达抑制了感染诱导的ROS积累。进一步的分析表明,PbrChiA可以与PbrLYK1b2的外结构域结合,这种相互作用抑制了PbrLYK1 b2介导的几丁质诱导的ROS积累。总之,我们提出,在后期坏死营养感染阶段,来自丰富的PbrChiA的较高抗真菌活性和较低的ROS水平相结合,赋予梨对B.dothidea的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
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