Development of Monoclonal Antibodies Specific to Either CD300A^R111 or CD300A^Q111 or Both.

Q3 Medicine

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Pub Date : 2023-10-01 DOI:10.1089/mab.2023.0017

Hitoshi Koizumi, Chigusa Nakahashi-Oda, Wenxin Lyu, Yumi Yamashita-Kanemaru, Keiji Tabuchi, Kazuko Shibuya, Akira Shibuya

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Abstract

CD300A is a member of the CD300 immunoglobulin (Ig)-like receptor family consisting of eight molecules in humans, all of which contain one Ig-like domain in the extracellular portion. Upon binding its ligand phosphatidylserine or phosphatidylethanolamine, CD300A mediates an inhibitory signal through the immunoreceptor tyrosine-based inhibitory motif in the cytoplasmic portion. The CD300 family molecules are highly homologous to each other. In addition, CD300A has a single nucleotide polymorphism (rs2272111), which is a nonsense mutation encoding glutamine (CD300A^Q111) instead of arginine (CD300A^R111) at residue 111 in the Ig-like domain of CD300A. In this study, we successfully generated monoclonal antibodies (mAbs) specific to either CD300A^R111 or CD300A^Q111 or both. These mAbs are useful for the analysis of CD300A genotype by flow cytometry and the development of an antibody drug for the treatment of various diseases.

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特异于CD300AR111或CD300AQ111或两者的单克隆抗体的开发。

CD300A是CD300免疫球蛋白（Ig）样受体家族的成员，该家族在人类中由八个分子组成，所有这些分子在细胞外部分都包含一个Ig样结构域。在结合其配体磷脂酰丝氨酸或磷脂酰乙醇胺时，CD300A通过细胞质部分中基于免疫受体酪氨酸的抑制基序介导抑制信号。CD300家族分子彼此高度同源。此外，CD300A具有单核苷酸多态性（rs2272111），这是在CD300A的Ig样结构域的残基111编码谷氨酰胺（CD300AQ111）而不是精氨酸（CD300AR111）的无义突变。在这项研究中，我们成功地产生了对CD300AR111或CD300AQ111或两者都有特异性的单克隆抗体（mAb）。这些mAb可用于通过流式细胞术分析CD300A基因型和开发用于治疗各种疾病的抗体药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy Medicine-Immunology and Allergy

CiteScore

4.80

自引率

0.00%

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